Ameliorative Efficacy of Phytochemical Content of Corchorus olitorius Leaves against Acute Ethanol-induced Oxidative Stress in Wistar Rats

Main Article Content

Augustine I. Airaodion
Emmanuel O. Ogbuagu
Ogbonnaya Ewa
Uloaku Ogbuagu
Olaide O. Awosanya
Olukule A. Adekale

Abstract

Aim: This study is aimed at investigating the ameliorative efficacy of methanolic extract of Corchorus olitorius leaves against acute ethanol-induced oxidative stress in Wistar rats.


Methods: Fresh plants of C. olitorius were harvested from the Institute of Agricultural Research and Training, Ibadan. The leaves were dried and extracted using soxhlet apparatus and methanol as the solvent. The methanol was evaporated in a rotary evaporator at 35°C with a yield of 2.17 g which represents a percentage yield of 8.68%. Twenty adult male Wistar rats with body weight between 120 and 150 g were used for this study. They were randomly divided into four groups of five rats each. Animals in groups 1 and 2 were administered saline solution while those in groups 3 and 4 were administered C. olitorius extract for twenty-one days. The animals were administered the extract and saline solution at a dose of 4 mL per 100 g body weight 12 hourly via oral route of administration. At the end of the treatment, they were fasted overnight and animals in groups 2 and 4 were exposed to a single dose of 70% ethanol at 12 ml/kg body weight to induce oxidative stress. After 12 hours of ethanol administration, the animals were anaesthetized using diethyl ether and were sacrificed. Liver was excised, weighed and homogenized in 50 mmol/L Tris–HCl buffer (pH 7.4) and then centrifuged at 5000 × g for 15 minutes for biochemical analysis. Supernatants were immediately kept frozen for further analysis.


Results: Ethanol-induced oxidative stress significantly increased the activities of AST, ALT, LDH, LPO, CAT, SOD and GPX but decrease GSH.  These effects were regulated by C. olitorius administration.


Conclusion: C. olitorius was able to remedy the effect of ethanol by regulating the oxidative stress biomarkers, thus possesses ameliorative efficacy against ethanol-induced oxidative stress and can protect the body against free radicals arising from oxidative stress.

Keywords:
Ameliorative efficacy, C. olitorius leaves, ethanol, oxidative stress.

Article Details

How to Cite
Airaodion, A., Ogbuagu, E., Ewa, O., Ogbuagu, U., Awosanya, O., & Adekale, O. (2019). Ameliorative Efficacy of Phytochemical Content of Corchorus olitorius Leaves against Acute Ethanol-induced Oxidative Stress in Wistar Rats. Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(2), 1-10. Retrieved from http://journalajbgmb.com/index.php/AJBGMB/article/view/30054
Section
Original Research Article

References

Adedosu OT, Akanni OE, Afolabi OK, Adedeji AL. Effects of Corchorus olitorius Extract on Certain Antioxidants and Biochemical Indices in Sodium Arsenite Exposed Rats. American Journal of Phytomedicine and Clinical Therapeutics. 2015;3(03):245-256

Onyeka EU, Nwambekwe IO. Phyto-chemical profile of some green leafy vegetables in South East, Nigeria. Nigeria Institute of Food Science and Technology; 2007.

Ujah OF, Ipav SS, Ayaebene CS, Ujah C. Phytochemistry and hepathoprotective effect of ethanolic leaf extract of Corchorus olitorius on carbon tetrachloride induced toxicity. European Journal of Medicinal Plants. 2014;4(8):882-892.

Nemba RM, Emadak A, Mouzong GC, Nemba CE. Qualitative and quantitative assessement of mineral elements in the leaves of Corchorus fascicularis and Corchorus olitorius harvested in Cameroon. J Curr Chem Pharm Sci. 2012;2(1):17-23.

Batran RA, Bayaty F, Abdulla MA, Obaidi MM, Hajrezae IM, Hassandarvish P, Fouad M, Golbabapour S, Talaee S. Gastro-protective effect of Corchorus olitorius leaf extract against ethanol-induced gastric mucosal hemorrhagic lesion in rats. J. gastroenterol hepatol. 2013;28(8):1321-1329.

Egua MO, Etuk EU, Bello SO, Hassan SW. Anti-diabetic activity of ethanolic seed extract of Corchorus olitorius. International Journal of Sciences: Basic and Applied Research. 2013;12(1):8-21.

Airaodion AI, Ogbuagu EO, Ogbuagu U, Awosanya OO, Airaodion EO. Effect of methanolic extract of Corchorus olitorius leaves on hypoglycemic and hypoli-pidaemic activities in albino rats. Asian Plant Research Journal. 2019;2(6):1-13.

Lieber CS. Ethnic and gender differences in ethanol metabolism, Alcohol. Clin. Exp. Res. 2000;24 (4):417–418.

Wu D, Cederbaum AI. Alcohol, oxidative stress, and free radical damage, Alcohol Res. Health. 2003;27:277–284.

Ronis MJ, Hakkak R, Korourian S, Albano E, Yoon S, Ingelman-Sundberg M, Lindros KO, Badger TM. Alcoholic liver disease in rats fed ethanol as part of oral or intragastric low-carbohydrate liquid diets. Exp. Biol. Med. 2004;229(4):351–360.

Gohlke JM, Griffith WC, Faustman EM. Computational models of ethanol-induced neurodevelopmental toxicity across species: Implications for risk assessment, Birth Defect Res. B: Dev. Reprod. Toxicol. 2008;83(1):1–11.

Lieber CS. Hepatic and other medical disorders of alcoholism: From pathogenesis to treatment, J. Stud. Alcohol. 1998;59(1): 9–25.

Nadro MS, Arungbemi RM, Dahiru D. Evaluation of Moringa oleifera leaf extract on alcohol-induced hepatotoxicity, Trop. J. Pharm. Res. 2007;5(1):539–544.

Das SK, Vasudevan DM. Alcohol-induced oxidative stress. Life Sci. 2007;81(3):177–187.

Rahman T, Hosen I, Islam MT, Shekhar HU. Oxidative stress and human health. Adv Biosci Biotechnol. 2012;3:997–1019.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4:118–126.

Gorinstein S, Yamamoto K, Katrich E, Leontowicz H, Lojek A, Leontowicz M, Trakhtenberg S. Antioxidative properties of Jaffa sweeties and grapefruit and their influence on lipid metabolism and plasma antioxidative potential in rats, Biosci. Biotechnol. Biochem. 2003;67(4): 907–910.

Ramana KV, Singhal SS, Reddy AB. Therapeutic potential of natural pharmaco-logical agents in the treatment of human diseases, Bio Med Res. Int. 2014;1–4.

Chikezie PC, Chiedozie O, Ibegbulem FN. Bioactive principles from medicinal plants. Res. J. Phytochem. 2015;9(3):88–115.

Morales-González JA. Oxidative stress and chronic degenerative diseases-A role for antioxidants. In Tech, Rijeka, Croatia. 2013;500.

Airaodion AI, Olatoyinbo PO, Ogbuagu U, Ogbuagu EO, Akinmolayan JD, Adekale OA, Awosanya OO, Agunbiade AP, Oloruntoba AP, Obajimi OO, Adeniji AR, Airaodion EO. Comparative assessment of phytochemical content and antioxidant potential of Azadirachta indica and Parquetina nigrescens leaves. Asian Plant Research Journal. 2019;2(3):1-14.

Airaodion AI, Ibrahim AH, Ogbuagu U, Ogbuagu EO, Awosanya OO. Akinmolayan JD, Njoku OC, Obajimi OO, Adeniji AR, Adekale OA. Evaluation of phytochemical content and antioxidant potential of Ocimum gratissimum and Telfairia occidentalis leaves. Asian Journal of Research in Medical and Pharmaceutical Sciences. 2019;7(1):1-11.

Airaodion AI, Obajimi OO, Ezebuiro CN, Ogbuagu U, Agunbiade AP, Oloruntoba AP, Akinmolayan JD, Adeniji AR, Airaodion EO. Prophylactic efficacy of aqueous extract of Curcuma longa leaf against indomethacin-induced ulcer. International Journal of Research. 2019;6(1):87-91.

Airaodion AI, Olayeri IM, Ewa AO, Ogbuagu EO, Ogbuagu U, Akinmolayan JD, Agunbiade AP, Oloruntoba AP, Airaodion EO, Adeniji AR, Obajimi OO, Awosanya OO. Evaluation of Moringa oleifera leaf potential in the prevention of peptic ulcer in wistar rats. International Journal of Research. 2019;6(2):579-584.

Airaodion AI, Ogbuagu U, Ogbuagu EO, Airaodion EO, Agunbiade AP, Oloruntoba AP, Mokelu IP, Ekeh SC. Investigation of aqueous extract of Zingiber officinale root potential in the prevention of peptic ulcer in albino rats. International Journal of Research and Innovation in Applied Science. 2019;4(2):64-67.

Airaodion AI, Adekale OA, Airaodion EO, Ogbuagu EO, Ogbuagu U, Osemwowa EU. Efficacy of combined crude extract of Curcuma longa and Moringa oleifera leaf in the prevention of peptic ulcer in albino rats. Asian Journal of Research in Medical and Pharmaceutical Sciences. 2019;7(2):1-9.

Han Y, Xu Q, Hu JN, Han XY, Li W, Zhao LC. Maltol, a food flavoring agent, attenuates acute alcohol-induced oxidative damage in mice, Nutrients. 2015;7(1):682–696.

Reitman S, Frankel S. A colorimetric method for determination of serum glutamate oxaloacetate and glutamic pyruvate transaminase. American Journal of Clinical Pathology. 1957;28:56-58.

Airaodion AI, Ogbuagu EO, Ogbuagu U, Adeniji AR, Agunbiade AP, Airaodion EO. Hepatoprotective effect of Parkia biglobosa on acute ethanol-induced oxidative stress in wistar rats. International Research Journal of Gastroenterology and Hepato-logy. 2019;2(1):1-11.

Saravanan R, Pari L. Antihyperlipidemic and antiperoxidative effect of diasulin, a polyherbal formulation in alloxan induced hyperglycemic rats. BMC Complement. Alternative Med. 2005;5:14–34.

Iseri OD, Yurtcu E, Sahin FI, Mehmet H. Corchorus olitorius (jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77). Pharmaceutical Biology. 2013;51(6):766-770.

Airaodion AI, Airaodion EO, Ewa O, Ogbuagu EO, Ogbuagu U. Nutritional and anti–nutritional evaluation of garri processed by traditional and instant mechanical methods. Asian Food Science Journal. 2019;9(4):1-13.

Airaodion AI, Airaodion EO, Ogbuagu EO, Ogbuagu U, Osemwowa EU. Effect of oral intake of African locust bean on fasting blood sugar and lipid profile of albino rats. Asian Journal of Research in Biochemistry. 2019;4(4):1-9.

Owoade AO, Adetutu A, Airaodion AI, Ogundipe OO. Toxicological assessment of the methanolic leaf extract of Bridelia ferrugelia. The Journal of Phytopharmaco-logy. 2018;7(5):419-424.

Brautbar N, Williams JII. Industrial solvents and liver toxicity: Risk assessment, risk factors and Mechanisms. International J of Hygiene and Environmental Health. 2002; 25(6):497-492.

Kaneko JJ, Harvey JW, Bruss ML. Clinical biochemistry of domestic animals. 5th Ed, Academic Press, London. 1999;829-44.

Jarrar BM, Mahmoud ZN. Histochemical demonstration of changes in the activity of hepatic phosphatases induced by experi-mental lead poisoning in male white rats (Rattus norvegicus). Toxicol Ind Health. 1999;15:1–9.

Gholipour-Kanani H, Shahsavani D, Baghishani H. Effect of exposure to sublethal levels of potassium cyanide on serum and tissue enzymes in roach fish (Rutilus rutilus). Online J Vet Res. 2013; 17(5):245-55.

Al-Ghanim KA. Effect of cypermethrin toxicity on enzyme activities in the freshwater fish Cyprinus carpio. African Journal of Biotechnology. 2014;13(10): 1169–1173.

Towle HC, Kaytor EN, Shih HM. Regulation of the expression of lipogenic enzymes by carbohydrates. Annual Review of Nutrition. 1997;17:405-433

Zubay G, Schwartz D, Beckwith J. Mechanism of activation of catabolite- sensitive genes: a positive control system. Proceedings of the National Academy of Sciences. 1970;66(1):104-110.

Todar K. Regulation and control of metabolism in bacteria: In: Todar's Online Textbook of Bacteriology. 2008;1-5.

Johnson PJ. The assessment of hepatic function and investigation of jaundice. In: Marshall WJ, Bangert SK, editors. Clinical Biochemistry – Metabolic and Clinical Aspects. Churchill Livingstone, New York. 1995;217 36.

Wright PJ, Plummer DT. The use of urinary enzyme measurement to detect renal damage caused by Nephrotoxic compounds. Biochem. Pharmacol. 1974;23:98-112.

Akanji MA, Olagoke OA, Oloyede OB. Effect of chronic consumption of meta-bisulphite on the integrity of the rat kidney cellular system. Toxicology. 1993;81:173-179.

Oyenihi OR, Afolabi BA, Oyenihi AB, Ogunmokun OJ, Oguntibeju OO. Hepato- and neuro-protective effects of watermelon juice on acute ethanol-induced oxidative stress in rats. Toxicology Reports. 2016;3: 288–294.

Tiwari AK, Rao JM. Diabetes mellitus and multiple therapeutic approaches of phyto-chemicals: Present status and future prospects. Curr. Sci. 2002;83:30-38.

Manzo-Avalos S, Saavedra-Molina A. Cellular and mitochondrial effects of alcohol consumption, Int. J. Environ. Res. Public Health. 2010;7(12):4281–4304.

Sathiavelu J, Senapathy GJ, Devaraj R, Namasivayam N. Hepatoprotective effect of chrysin on prooxidant-antioxidant status during ethanol-induced toxicity in female albino rats, J. Pharm. Pharmacol. 2009; 61(6):809–817.

Ramezani A, Goudarzi I, Lashkarboluki T, Ghorbanian MT, Abrari K, Salmani ME. Role of oxidative stress in ethanol-induced neurotoxicity in the developing cerebellum, Iran. J. Basic Med. Sci. 2012;15(4):965.

Agarwal DP. Genetic polymorphisms of alcohol metabolizing enzymes, Pathol. Biol. 2001;49(9):703–709.

Orieke D, Ohaeri OC, Ijeh II, Ijioma SN. Identification of phytocomponents and acute toxicity evaluation of Corchorus olitorius leaf extract. European Journal of Medicinal Plants. 2018;23(1)1-16.

Oh SI, Kim CI, Chun HJ, Park SC. Chronic ethanol consumption affects glutathione status in rat liver, J. Nutr. 1998;128(4): 758–763.

Murray RK, Granner DK, Mayes PO, Rodwell VW. Harper's illustrated biochemistry. 26th Edn., Appleton and Lange Medical Publication/McGraw Hill, USA. 2003;693.
[ISBN-13: 9780071389013]

Halliwell B, Gutterberidge JMC. Free radicals in biology and medicine. 4th ed. Oxford, UK: Oxford University; 2006.

Yang SS, Huang CC, Chen JR, Chiu CL, Shieh MJ, Lin SJ, Yang SC. Effects of ethanol on antioxidant capacity in isolated rat hepatocytes, World J. Gastroenterol. 2005;11(46):7272.

Shirpoor A, Minassian S, Salami S, Khadem-Ansari MH, Yeghiazaryan M. Alpha-lipoic acid decreases DNA damage and oxidative stress induced by alcohol in the developing hippocampus and cerebellum of rat, Cell. Physiol. Biochem. 2008;22:(5–6):769–776.