Evaluation of Antimalarial Properties of Ficus platyphylla Del Leaf Extract in Mice

Main Article Content

J. Omame
A. E. Amobonye
A. Y. Kabiru

Abstract

Aim: This study was aim at investigating the effect of crude petroleum ether leaf extract of Ficus platyphylla Del on Plasmodium berghei infected mice.

Place and Duration of Study: This research was carried out at the department of biochemistry, Federal university of technology minna, Niger state Nigeria in 2014.

Methodology: The crude plant extract of F. platyphylla was administered 72 hours at different doses post and pre infection for both the curative and prophylactic study respectively against residual infection. Mice were divided into 5 groups of 5 mice each, 3 of the groups where administered crude plants extract of F. platyphaylla at different doses (200, 400 and 600 mg/kg body weight) while the other two serve as negative and positive control group and were administered 0.5 ml and 50 mg/kg body weight respectively.

Results: The extract at all doses produced significant (P<0.05) dose dependent chemo-suppressive activity with %  inhibition of 38%, 61%, 74% and 81.8% for curative studies and 36.0%, 38.5%, 49.5% and 63.4% for prophylactic studies against the parasites at doses of 200 mg/kgbw, 400 mg/kgbw, 600 mg/kgbw of the extract and 50 mg/kgbw of Artesunate. All doses of the extract increased the survival time of the infected mice compared to the negative control group that was administered 0.5 ml normal saline. The variation in the values of Packed Cell Volume (PCV) for treated group before and after extract administration was not significant at (P<0.05). The phytochemical screening of the plant extract showed the presence of tannin, saponin, flavonoids, terpenoids, steroids, anthroquinone and phenol.

Conclusion: The result of this study shows that F. Platyphylla leaf extract exhibited some antiplasmodial activity that could be exploited for safe, effective and affordable antimalaria regimen.

Keywords:
Malaria, Ficus platyphylla, Plasmodium berghei, anti-malarial, suppressive.

Article Details

How to Cite
Omame, J., Amobonye, A. E., & Kabiru, A. Y. (2019). Evaluation of Antimalarial Properties of Ficus platyphylla Del Leaf Extract in Mice. Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(2), 1-8. https://doi.org/10.9734/ajbgmb/2019/v2i230057
Section
Original Research Article

References

World Health Organisation (WHO). Geneva, world malaria report, Global Malaria Programme; 2011.

World Health Organization, World Malaria Report. Geneva, Global Plan for Insecticide Resistance Management in Malaria Vectors (GPIRM). 2011;13.

Lamar J, Martschinske R, Tetreault G, Doud C. Navy medical department pocket guide to malaria prevention and control. 2007;1-112.

Teinkela JEM, Noundou XS, Nguemfo EL, Meyer F, Wintjens R, Isaacs M, Azebaze AGB. Biological activities of plant extracts from Ficus elastica and Selaginella vogelli: An antimalarial, antitrypanosomal and cytotoxity evaluation. Saudi Journal of Biological Sciences. 2018;25(1):117-122.

Apampa Sulaiman Ayodeji, Adoum Oumar Al-Moubarak, Fidelis Solomon Ameh. Antimalarial activity of Alysicarpus zeyheri (Harv), Borreria scabra, Uapaca pilosa (Hutch) Extracts on mice infected With Plasmodium berghei. European Journal of Biomedical and Pharmaceutical Sciences. 2019;6(1):607-614

Maroyi A. Traditional use of medicinal plants in south-central Zimbabwe: Review and perspectives. Journal of Ethnobiology and Ethnomedicine; 2013.

Nadembega P, Boussim JI, Nikiema JB, Poli F, Antognoni F. Medicinal plants in baskoure, kourittenga province, Burkina Faso: an ethnobotanical study. Journal of Ethnopharmacology. 2011;133(2):378–395.

Chindo B, Joseph A, Amos A. Anticonvulsant properties of Saponins from Ficus platyphylla Stem Bark. Brain Research Bulletin 2009;78(6):276-282.

Chindo BA, Amos S, Odutola AA, Vongtau HO, Abbah J, Wambebe C, Gamaniel KS. Central nervous system activity of the methanol extract of Ficus platyphylla stem bark. Journal of Ethnopharmacological. 2003;85:131–137.

Jigam AA, Usman TA, Halima AM, Tijani FO. Efficacy of thonningia sanguine Vahl root extract against Plasmodium chabaudi, inflammation and nociception in mice. Journal of Applied Pharmaceutical Science. 2012;02(01);47-51.

Canadian Council of Animal Care (CCAC) Guidelines and Protocol Review; 1997.

Kabiru AY, Okolie NL, Muhammad HL, Ogbadoyi EO. Preliminary studies on the antiplasmodial potential of aqueous and methanol extracts of Eucalyptus camaldulensis. Asian Pacific Journal of Tropical Disease. 2012;S809-S814.

Ogbadoyi EO, Akinsunbo OA, Adama TZ, Okogun JI. In vivo trypanocidal activity of Annona senegalensis Pers. leaf extract against Trypanosoma brucei brucei. Journal of Ethnopharmacology. 2007;112: 85-89.

Odebiyi OO, Sofowora ES. Antimicrobial alkaloids from a Nigerian chewing stick (Fagaraz anthoxyloides). Planta Medicals. 1979;36:204–207.

Ryley JF, Peters W. The antimalarial activity of some quinone esters. Annals of Tropical Medicine and Parasitology. 1970; 84:209–222.

Saidu K, Onah J, Orisadipe A, Olusola A, Wambebe C, Gamaniel K. Antiplasmodial, analgesic and anti-inflammatory activities of the aqueous extract of the stem bark of Erythrina senegalensis. Journal of Ethnopharmacology. 2000;71: 275–280.

White N, Nosten F, Bjorkman A, Marsh K, Snow RW. The Lancet. 2004; 363:1160.

Daice JV, Lewis SM. Practical Haematology, 5th Edition, Churchill-living stone, Edinburgh; 1975.

Tshibangu JN, Chifundera K, Kaminsky R, Wright AD, Konig GM. Screening of Af-rican medicinal plants for antimalarial inhibitory activity. Journal of Ethnopharmacology. 2002;80:25-35.

Ugwah-Oguejiofor CJ, Bello SO, Etuk EU, Igbokwe VU, Ugwah OM, Okolo RU. Preliminary toxicity and phytochemical studies of the aqueous extract of Ficus platyphyllain female albino rats. International Research Journal of Pharmacy and Pharmacology. 2011;1(5): 086- 092.

Olugbenga AM, Musa AE, Oluwatoyin AH. Toxicological activity of crude saponin extract of Ficus platyphylla. Asian Journal of Pharmaceutical and Clinical Research. 2012;5(1):30-33.

Okokon J, Ofodum KC, Ajibesin KK, Danlandi B, Gamaneil KS. Pharmacological screening and evaluation of antiplasmodial activity of Croton zambesicus against P. berghei infection in mice. Indian Journal of Pharmacology. 2005;37:243-246.

Salawu OA, Tijani AY, Babayi H, Nwaeze AC, Anagbogu RA, Agbakwuru VA. Anti- malaria activity of ethanolic stem bark extract of Faidherbia albida (Del) a. Chev (Mimosoidae) in mice. Archives of Applied Science Research. 2010;2(5):261-268.

Shittu I, Emmanuel A, Nok AJ. Antimalaria effect of the ethanolic stem bark extracts of Ficus platyphylla del. Journal of Parasitological Research. 2011;10(5): 1155- 1160.

Esievo KAN, Saror DL, Ilemobade AA, Hallaway AM. Variation in erythrocytes surface and free serum sialic acids concentration during experimental trypanosome vivax infection in cattle. Research in Vet Sci. 1982;32(1):1-5.

Kabiru AY, Abdulkadir A, Gbodi AT, Bello UM, Makun HA, Amah DJ, Ogbadoyi EO. Evaluation of haematological changes in plasmodium-berghei- infected mice administered with aqueous extract of phyllantus amarus. Pakistan Journal of Biological Science. 2013;16(11):510-516.