Genetic Variability and Diversity Analysis in Pod and Seed Characters of Some Neglected and Underutilized Legumes (NULs)

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J. O. Agbolade
T. P. Olakunle
K. M. Popoola
J. A. Idowu
A. I. Isiaka
A. D. Aasa-Sadique


In response to the paucity of information challenge on the neglected and underutilized legumes, this paper explored pods and seeds morphological data of the twenty-four accessions of these crops with a view to establishing the occurrence of genetic variability and diversity analysis among the studied taxa. Twenty-four accessions of neglected and underutilized legumes (NULs) obtained from International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria were assessed for genetic variability and diversity analysis through Pod and seed morphological characters. Each accession was planted into plot of 5 ridges of 5 meter long, spaced 1 meter apart and replicated three times at the teaching and research farm of the Federal University Oye Ekiti, Ekiti State, Nigeria. Descriptive statistics was employed to evaluate differences in the mean values of the accessions while discriminatory traits among accessions were identified by Principal Component Analysis (PCA). Similarities among the studied plants were assessed by cluster analysis. The first two principal component axes explained 72% of the total variation. Pod length, pod width and 100-seed weight were traits that contributed most of the variations in the legume accessions. There was a display of intra-species similarities and inter-specific genetic diversity among the studied accessions.

Variability, diversity, analysis, pod and seed, NULs, PCA.

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How to Cite
Agbolade, J. O., Olakunle, T. P., Popoola, K. M., Idowu, J. A., Isiaka, A. I., & Aasa-Sadique, A. D. (2019). Genetic Variability and Diversity Analysis in Pod and Seed Characters of Some Neglected and Underutilized Legumes (NULs). Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(3), 1-8.
Original Research Article


Klu GYP, Amoatey HM, Bansa D, Kumaga FK. Cultivation and use of African yam bean (Sphenostylis stenocarpa) in the Volta Region of Ghana. The Journal of Food Technology in Africa. 2001;6:74-77.

Adebowale SA, Lawal OG. Comparative study of the functional properties of bambara groundnut, jack bean and mucuna bean flours. Food Res. Int. 2004;27:355-365.

Adewale BD, Okonji C, Oyekanmi AA, Akintobi DAC, Aremu CO. Genotypic variability and stability of some grain yield components of cowpea. African Journal of Agricultural Research. 2010b;5:874-880.

Adewale BD, Odoh NC. A review on genetic resources, diversity and agronomy of African yam bean (Sphenostylis stenocarpa (Hochst. Ex A. Rich.) Harms): A potential future food crop. Sustainable Agr. Res. 2013;5:32-38.

Nnamani CV, Ajayi SA, Oselebe HO, Atkinson CJ, Igboabuchi AN, Ezigbo EC. Sphenostylis stenocarpa (ex.A.Rich.) Harms., a fading genetic resource in a changing climate: Prerequisite for conservation and sustainability. Plants (Basel). 2017;6.

Agbolade JO, Popoola JO, Kioko JI, Adewale BD, Ajiboye AA, Ajewole TO, David OA, Komolafe RJ. Comparative genetic variability and traits’ heritability in vegetative and floral characters in accessions of two minor legumes. Indian Journal of Agricultural Research. 2019;53:198-183.

Agbolade JO, Olakunle TP, Aina DA, Adeyemo IA, Aasa-Sadique AD, Taiwo JO. Morpho-genetic vegetative variabilities of some underutilized legumes. J. Pharm. Sci. 2013;2(8).

King HG. Pasture for the South, 4th Edition. The Inter Estate Printers and Publishers, Inc. Daneville III. 1963;1-12.

Agbolade JO, Komolafe RJ. Genetic diversity and phylogenetic behaviour of some minor legumes. Inter. J. Plant Biol. 2016;7:15–21.

Richard. Replicator selection and the extended phenotypes. Ethology. 1978;47:61-76.

Churchhill FB. Willaiam Johannsen and the genotype concept. Journal of the History of Biology. 1974;7:5-30.

Ammar MH, Alghamdi SS, Migdadi HM, Khan MA, El-Harty EH, Al-Faifi SA. Assessment of genetic diversity among faba bean genotypes using agro-morphological and molecular markers. Saudi J Biol Sci. 2015;22:340-50.

Esquinas-Alcazar J. Protecting crop genetic diversity for food society: Political, ethical and technical challenges. Nat. Rev. Genet. 2005;6:946-953.

Karikari SK. Variability between local and exotic groundnut landraces in Botswana. African Crop Science Journal. 2000;8:145-152.

Ofosu IW, Elis WO, Nsiah K, Oduro IN. NULs: Exposure assessment, habitual eating and cooking habits and consumers’ characteristics. Journal of Food Security. 2017;5:169-175.

Padulosi S, Hodgkin T, Williams JT, Hag N. Underutilized crops: Trends, challenges and opportunities on 21st century. In: J.M.M., E.,V.R, RAO, A.H.D. Brown, M.T. Jackson (Eds), Managing Plant Genetic Diversity. CABI International, Wallingford, UK; 2000.

Ariyo OJ. Genetic diversity in West Africa Okro (Abelmoschus caillei)-Multivariate analysis of morphological and agronomic characters. Genetic Resources and Crop Evolution. 1993;40:25-32.

Chedda HR, Fatokun CA. Numerical taxonomy of variation pattern in Okro (Abelmoschus caillei (L.) Moench). Botanical Gazzete. 1982;143:253- 261.

Popoola JOA, AE, Obembe OO, Agbolade JO. Reproductive mechanisms and pollen characterization in some accessions of an underutilized legume: (Sphenostylis stenocarpa Hochst Ex. A. Rich) harms. International Journal of Biodiversity and Conservation, Academic Journals. 2011;25:185-192.