Therapeutic Effects of Viscum album Combined with Garcinia kola against CCL4 Induced Liver Injury in Albino Rats

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Roseline Emeji
Tamuno-Emine Davies Gabriel
Bosia Ndokiari


This study was aimed at evaluating the efficacy of Viscum album (mistletoe) and Garcinia kola seed (bitter kola) in treating Rat liver against CCl4-induced liver injury. Mistletoe and bitter Kola are used in traditional medicine for the treatment of various disorders, including hepatic disorders. Biochemical parameters and histological structure were assessed and used as a measure of the therapeutic potential of the herbs against CCl4 induced liver injury. The experimental animals (15 male wistar albino rats) weighing between 100-120 g were randomly divided into three (3) groups. Each group comprised 5 rats and was labeled as group 1, 2 or 3. Group 1 (negative control) animals were administered saline orally daily for 6 weeks (1 ml volume per kg body weight) while group 2 (CCl4 group) animals were administered CCl4 mixed with olive oil as vehicle in 1:1 ratio (3 ml/kg body weight). Group 3 represented the treatment group with extracts of the two herbal plants (250 mg/kg daily). The combination of the herbal extracts administered orally for 6weeks showed a significant decrease (P<0.05) in serum protein, albumin, total bilirubin and conjugated bilirubin concentrations and in serum activities of AST, ALT, ALP and GGT compared with CCl4-induced increases in concentrations or activities of the aforementioned biochemical parameters. Histological examination of the liver of CCl4–treated rats with the combined herbal extracts showed less destruction of liver architecture in comparison to the group induced with CCl4 only. The results indicated that the combination herbal extracts investigated (mistletoe and bitter kola) had therapeutic effect against CCL4-induced liver injury when used either in combination and this effect could be due to the phytochemicals present in the herbs.

Therapeutic effects, Viscum album, Garcinia kola, CCL4 induced, liver injury.

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How to Cite
Emeji, R., Gabriel, T.-E., & Ndokiari, B. (2019). Therapeutic Effects of Viscum album Combined with Garcinia kola against CCL4 Induced Liver Injury in Albino Rats. Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(3), 1-8.
Original Research Article


Chang FY, Lee SD. Cytochrome P450 2E1 genotype and the susceptibility to antituberculosis drug-induced hepatitis. Hepatology. 2003;37:924–930.

Hummer M, Kury M, Kurzthaler I, Oberbauer H, Miller C, Fleischhacker W. Hepatotoxicity of clozapine. Journal of Clinical Psychopharmacology. 1997;17: 314-317.

Jenner AM, Timbrell JA. In vitro microsomal metabolism of hydrazine. Xenobiotica. 1995;25:599–609.

Devanathan V, Preethi R, Loganathan M. Antimicrobial and antioxidant efficacy of some medicinal plants against food borne pathogens. Advances in Biological Research. 2010;4:122-125.

Akintowa A, Essien AR. Protective effects of Garicinia kola Seed extract against paracetamol-induced hepatotoxicity in rats. Journal of Enthnopharmacology. 1990;29(2):207-211.

Tona L, Ngmibi NP, Tasakala M, Mesiak K, Cimanga K, Apers S. Antimalarial activity of extract of 20 crude extracts from nine African medicinal plants used in Kinshasa, Congo. Journal of Ethanopharmacology. 1999;15(68):193-203.

Farombi EO. Mechanisms for the hepato protective action of kolaviron studies on hepatic enzymes, microtonal lipids and lipid per oxidation in carbon tetrachloride treated rats. Pharmacological Research. 2000;42:75-80.

Pietta PG. Flavonids as atioxidant. Journal of Natural Products. 2000;63(7):1035-1042.

Okunji CO Tantalia AW, Hicks RP, Iwu MM, Skanchy DJ. Capillary electrophoresis determination of medicinal formulations. Plant Medicine, 2002;68:440-444.

Farombi EO, Okanni OO, Emerole GO. Anti oxidative and sweetening activities of Kolaviron in vitro. Pharmaceutical Biology. 2002;40:107-116.

Adejoke EM, Ibukun DA, Erommonselle E, Adam MA. Hepatoprotective effects of Garcinia kola (bitter kola) against paracetamol-induced oxidative damage and glyconge degranulation in hapatocytes of adult male wistar rats. Journal of Advances in Biology and Biotechnology. 2015;3(3):110-116.

Aniche GN, Uwakwe GU. Potential use of Garcinia kola as hop substitute in larger beer brewing. World Journal of Microbiology and Biotechnology. 1990;6:323-486.

Ogu EO, Ahu RC. A comparison of some chemical properties of Garcinia kola and hops for assessment of Garcinia brewing values. Bioresource Technology. 1995;54: 1-4.

Eleyinmi AF, Oloyo RA. Pilot scale brewing trials using formulated blends of selected local vegetables as hop substitute. Journal of Food Science & Technology. 2001;38(6):609-611.

Eleyinmi F, Amoo IA, Oshodi AA. Antimicrobial activity of aqueous extracts of Vernonia amygdalina, Garcinia kola and Gongronema latifolium and their blends on some beer spoilage organisms. Technical Quarterly of the Master Brewer’s Association of the Americans. 2004;41(4):398-402.

Adeyeye EI. The relative merits of the presence of hull on the nutritional qualities of the african yam bean flour. Nahrung. 1998;42:84-88.

Firson-Norrie S, Sporns P. Identification and quantification of flavonol glycosides in almond seedcoats using MALDI-TOF MS. Journal of Agricultural and Food Chemistry. 2002;50(10):2782-2787.

Emeji Roseline, Boisa N, Tamuno-Emine DG. Therapeutic effect of Viscum album against ccl4 induced hepatoxicity in male albino rats. World Journal of Pharmaceutical and Medical Research. 2018;4(7):14-20.

Osadebe PO, Ukwueze SE. A comparative study of the phytochemical and anti-microbial properties of the Eastern Nigerian specie of African mistletoe (Loranthus micranthus) sourced from different host trees. Bio-Research, 2004;2 (1):18-23.

Nyblom H, Berggren U, Ballidin J, Olsson R. High AST/ALT ratio may indicate advanced alcoholic liver disease rather than heavy drinking. Alcohol and Alcoholism. 2004;39(4):336-339.

Jendrassik L, Grof P. A colorimetry method for determination or serum bilirubin. Biochemistry. 2006;1:297-81.

Kafaru E. Mistletoe-An example of an All-purpose Herb Herbal Remedies. In: Guardian Newspaper; 2003.

Obatomi DK, Aina VO, Temple VJ. Effect of African mistletoe on blood pressure in spontaneously hypertensive rats. Inter-national Journal of Pharmacognosy. 2006;34(2):124-127.

Oyetayo VO, Osho B. Assessment of properties of strain of Lactobacillus & Plantarum isolated from fermentry corn slurry (OGI). Journal of Food Agriculture and Environment. 2004;2(1):132-134.

Alade A, Ani RE. Protective effects of Garcinia kola seed extract against paracetamol-induced hepatotoxicity in rats. Journal of Enthopharmacology. 1990; 29(2):207-211.

Osifo UC, Akpamu U, Otamere HO, Ekhator CN. A Murine model study on the effect of Garcinia kola on body weight. Archives of Applied Science Research. 2011;3(5):526-531.

Galam NZ, Gambo IM, Habeeb AA, Shugaba AI. The effect of aqueous extract of Garcinia kola seed on the liver. Journal of Natural Sciences Research. 2013;3(1): 81-87.