Isolation and Identification of Microorganisms Growing on the Root of Leguminous Plants (Groundnut, Soya bean and Pea)

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Bukola Catherine Akin-Osanaiye
Oluwatobi Olaife Arowolo
Ifeyomi Wilfred Olobayotan


Study on the isolation and identifications of bacteria associated with the root of legumes were conducted using Spread Plate Technique. The frequencies of occurrences of the bacteria isolate showed that a total of sixteen (16) bacteria belonging to three genera and four species were isolated from the leguminous plants. Maximum number recovered from sample collected from the root of groundnut was seven (7) followed by Soya bean with five (5) while Pea recorded the least number of four (4). Role of Bacillus subtilis in the soil around the leguminous plant was the highest, which covered about 37.50% of the total isolates. Other bacteria that were also isolated from the soil around the legumes root include Bacillus cereus and Staphylococcus aureus which covered about four (4) each representing 25.0% of the total isolates while Pseudomonas aeruginosa recorded the least value of 12.50%. The bacteria isolated from the root of the legumes were not significantly different (P < 0.05). The bacteria have Nitrogen-fixing potential, having isolated from three leguminous plants which include Soya bean, Groundnut and Pea.

Isolation, identification, leguminous, nitrogen-fixing.

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Akin-Osanaiye, B. C., Arowolo, O., & Olobayotan, I. W. (2019). Isolation and Identification of Microorganisms Growing on the Root of Leguminous Plants (Groundnut, Soya bean and Pea). Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(3), 1-7. Retrieved from
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Barea JM, Pozo MJ, Azcon R, Azcon-Aguilar C. Microbial co-operation in the rhizosphere. Journal of Experimental Botany. 2005;56:1761-1778.

Hinsinger P, Bengough AG, Vetterlein D, Young IM. Rhizosphere: Biophysics, biogeochemistry and ecological relevance. Plant Soil Plant and Soil. 2009;321(1-2): 117-152.

Bashan Y, Holguin G. Proposal for the division of plant growth-promoting rhizobacteria into two classifications: Biocontrol-PGPB (plant growth-promoting bacteria) and PGPB. Soil Biology and Biochemistry. 1998;30:1225-1228.

Versalovic J, Schneider M, De Bruijn FJ, Lupski JR. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Cell Biology. 1994;5:25-40.

Glick BR. The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology. 1995;41:109- 114.

Han J, Sun L, Dong X, Cai Z, Sun X, Yang H, Wang Y, Song W. Characterization of a novel plant growth-promoting bacteria strain, Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens. Systemic and Applied Microbiology. 2005;28:66-76.

Ahmad F, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth-promoting activities. Microbiology Research, 2008;163:173- 181.

Misko AL, Germida JJ. Taxonomic and functional diversity of pseudomonads isolated from the roots of field grown canola. Microbial Ecology. 2002;42:399-407.

Dunfield KE, Germida JJ. Diversity of bacterial communities in the rhizosphere and root interior of fieldgrown genetically modified Brassica napus. Microbiology Ecology. 2001;38:1-9.

Sylvia D, Fuhrmann J, Hartel P, Zuberer, D. Principles and applications of soil microbiology. pearson education Inc. New Jersey. 2005;23-25.

Nihorimbere V, Ogena M, Thonart P. Beneficial effect of the rhizosphere microbial community for plant growth and health. Science of The Total Environment. 2011;15: 5-11.

Kent AD, Triplett EW. Microbial communities and their interactions in soil and rhizosphere ecosystems. Annual Review of Microbiology, 2002;56:211-236.

Giongo, Adriana, Anelise Beneduzi, Adriana Ambrosini, Luciano Kayser Vargas, Marcos Roberto Stroschein, Flávio Luiz Eltz, Maria Helena Bodanese-Zanettini, Luciane Maria Pereira Passaglia. Isolation and characterization of two plant growth-promoting bacteria from the rhizoplane of a legume (Lupinus albescens) in sandy soil. Revista Brasileira de Ciência do Solo. 2010;34(2):361-369.

Geetha R, Maheshwari HP, Sanket JJ. Isolation and characterization of nodule-associated Exuguobacterium sp. from the root nodules of Fenugreek (Trigonella foenum-graecum) and their possible role in plant growth promotion. International Journal of Microbiology, 2012;55:1-8.

Chesebrough M. Medical laboratory manual for tropical countries. Second edition, University Press, Cambridge, Great Britain, 2006;11:377.

Sturz AV, Christie BR, Matheson BG, Nowak J. Biodiversity of endophytic bacteria which colonize red clover nodules, roots, stems and foliage and their influence on host growth. Biology and Fertility of Soils. 1997;25(1):13–19.

Mishra J, Singh R, Arora NK. Plant growth-promoting microbes: diverse roles in agriculture and environmental sustainability. Krumar V, Kumar M, Sharma S, Prasad R. (Eds.). Probiotics and Plant Health, Springer. 2017;71–111.

Bai Y, D’Aoust F, Smith DL, Driscoll BT. Isolation of plant-growth-promoting Bacillus strains from soybean root nodules. The Canadian Journal of Microbiology. 2002;48(3):230–238.