Identification of Fusarium spp. Associated with Potato Tubers in Upper Egypt by Morphological and Molecular Characters

Main Article Content

Youssuf A. Gherbawy
Mohamed A. Hussein
Eman G. A. El-Dawy
Nabila A. Hassany
Saad A. Alamri

Abstract

Potato (Solanum tuberosum L.) is an important crop which holds promise for food security considering the global population growth rate. Fusarium dry rot is one of the most significant diseases of potato. To build up strategies for the control of this disease, it ought to be made primarily a correct diagnosis and identification of the pathogen. A total of 504 Fusarium isolates were recovered from potato tubers collected from Upper Egypt. Fusarium isolates were identified based on morphology and partial DNA sequencing of β-tubulin (TUB) genes. 62.5% of the isolates were identified as F. sambucinum, followed F. oxysporum (57.5%), then F. verticillioides (56.25%) and F. incarnatum (47.5%). All the tested Fusarium species were able to produce amylase. The pathogenicity of the isolates was tested by inoculation of healthy potato tubers; all of the tested isolates were pathogenic to healthy potato tubers. F. sambucinum had a highly virulent effect.

Keywords:
Potato (Solanum tuberosum L.), Fusarium spp., pathogenicity, amylase.

Article Details

How to Cite
Gherbawy, Y. A., Hussein, M. A., El-Dawy, E. G. A., Hassany, N. A., & Alamri, S. A. (2019). Identification of Fusarium spp. Associated with Potato Tubers in Upper Egypt by Morphological and Molecular Characters. Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(3), 1-14. https://doi.org/10.9734/ajbgmb/2019/v2i330062
Section
Original Research Article

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