Biochemical Characterization on Photosynthetic Activities during Dark Incubated Senescence of Wheat Primary Leaves by Gibberellic Acid

Main Article Content

G. Fareeda
S. D. S. Murthy


In annual crop plants like maize, rice and wheat etc. Senescence limits crop yields of annual crops like maize rice and wheat. Delayed leaf senescence is a desirable agronomic trait to improve crop yield. In this study 10 µM GA reduced the loss of wheat primary leaves under incubated dark conditions. GA reduced the loss of pigments, proteins, electron transport activities, spectral properties. The restoration of WCE activity by GA was closely associated with the restoration of PS II activity compared to that of PS I. GA treated leaf thylakoid membranes showed an increase in absorption at 680 nm moderate increase at 480 nm and 440 nm at 72 h during dark incubation. GA protected the degradation of water oxidation complex polypeptides (33, 23, 17 KDa) of PS II and slightly protected the PS I polypeptides.

GA, PS II activity, electron transport chain, polypeptide analysis, senescence.

Article Details

How to Cite
Fareeda, G., & Murthy, S. D. S. (2019). Biochemical Characterization on Photosynthetic Activities during Dark Incubated Senescence of Wheat Primary Leaves by Gibberellic Acid. Asian Journal of Biochemistry, Genetics and Molecular Biology, 2(4), 1-5.
Original Research Article


Patel KG, Ramana Rao TV. Effect of stimulated water stress on the physiology leaf senescence in three genotypes of cowpea. Indian Journal of Plant Physiology. 2007;12:138-145.

Moore B, Zhou L, Rolland F, Hall Q, Cheng WH, Liu YX, Hwang L, Jones T, Sheen, J. Role of arabidopsis glucose sensor HXK1 in nutrient, light and hormonal signaling. Science. 2003;300: 332-336.

Arnon DI. Copper enzymes in isolated chloroplasts. Poly phenol oxidase in Beta vulgaris. Plant Physiol. 1949;24:1-15.

Lowry OH, Rosebrought JH, Farr AL, Randall RJ. Protein measurement with the poly phenol reagent. J. Biol. Chem. 1951; 193:265-275.

Saha S, Good NE. Products of the photophosphorelation reactions. J. Biol. Chem. 1970;245:5017-5021.

Swamy PM, Murthy SDS, Suguna P. Retardation of dark induced in vitro alterations in PS II organization of cowpea leaf discs by combination of Ca2+ and benzyladenine. Boil. Plant. 1995;37:457-460.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227: 680-685.

Fletcher RA, Osborne DJ. Regulation of protein and nucleic acid synthesis by gibberellin during leaf senescence. Nature. 1965;207:1176-1177.

Prakash JSS, Baig MA, Mohanthy P. Age dependent alteration in PSII acceptor side in Cucumis sativus cotyledonay leaf thylakoids: Analysis of binding characteristics of herbosides (C 14) Atazin. Indian J. of Biophys. 1999;36:10-13.

Atal N, Pardhasaradhi P, Mohanthi P. Inhibition of the chloroplast photochemical reactions by treatment of wheat seedlings with a low concentration of cadmium. Analysis of electron transport activities and changes in fluorescence yield. Plant Cell Physiol. 1991;32:943-951.