Antibacterial Potential of Ethanolic and Aqueous Extracts of Carica papaya Leaves

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Augustine I. Airaodion
John A. Ekenjoku
Ime U. Akaninyene
Anthony U. Megwas


Background: The search for newer sources of antibiotics is a global challenge pre-occupying research institutions, pharmaceutical companies and academia, since many infectious agents are becoming resistant to synthetic drugs.

Aim: This present study sought to investigate the antibacterial potential of ethanolic and aqueous extracts of Carica papaya leaves.

Materials and Methods: Fresh and healthy leaves of C. papaya were harvested, air dried and milled into powder. The powder was extracted using ethanol and water as solvents. The antibacterial activities of both extracts were determined by diffusion method. Nutrient agar medium was prepared using standard method. Pure cultures of Coliform bacillus, Staphylococcus epidermidis, Streptococcus viridans, Salmonella typhi and Escherichia coli were obtained from the Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, Nigeria. The extracts were serially diluted to obtain 1.0%, 0.5%, 0.25% and 0.125% solutions in sterile test tubes. Sterilized 9 mm filter paper disc soaked in the diluted extracts were placed on the plate and incubated for 24 hours at room temperature. The plates were examined for clear zones of inhibition. Presence of zones of inhibition indicated activity.

Results: the results showed that both ethanolic and aqueous extracts of C. papaya leaves exhibit antibacterial activities against C. bacillus, S. epidemidis, S. viridans and E. coli and also inhibited their growth. The effect of the ethanolic extract was greater than that of the aqueous extract. However, this activity was not observed with S. typhi.

Conclusion: The result of the present study showed that C. papaya leaves might effectively inhibit the growth of C. bacillus, S. epidemidis, S. viridans and E. coli but not that of S. typhi. However, the ethanolic extract is more potent than the aqueous extract.

Carica papaya, C. bacillus, E. coli, S. epidemidis, S. typhi, S. viridians

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How to Cite
Airaodion, A. I., Ekenjoku, J. A., Akaninyene, I. U., & Megwas, A. U. (2020). Antibacterial Potential of Ethanolic and Aqueous Extracts of Carica papaya Leaves. Asian Journal of Biochemistry, Genetics and Molecular Biology, 3(3), 33-38.
Original Research Article


Timothy O, Idu M. Preliminary phytochemistry and in vitro antimicrobial properties of aqueous and methanol extracts of Icacina trichantha. Oliv. Leaf. 2011;1(3):184–188.

Ikram M, Inamul H. Screening of medicinal plants for antimicrobial activities. Fitoterapia. 2013;55:62-64.

Izzo AA, Di Carlo G, Biscardi D, Fusco R, Mascolo N, Borreli F, Capasso F, Fasulo MP, Autore G. Biological screening of Italian medicinal plants for antibacterial activity. Phytother. Res. 2015;9:281-286.

Bibitha B, Jisha VK, Salitha CV, Mohan S, Valsa AK. Antimicrobial activity of different plant extracts. Short Communication. Indian Journal of Microbiology. 2002;42: 361–363.

Munoz V, Sauvain M, Bourdy G, Callapa J, Rojas I, Vargas L. The search for natural bioactive compounds through a multidisciplinary approach in Bolivia Part II. Antimalarial activity of some plants used by Moseteneindians. Journal of Ethno-pharmacology. 2000;69:139-155.

Mello VJ, Gomes MT, Lemos FO, Delfino JL, Andrade SP, Lopes MT, et al. The gastric ulcer protective and healing role of cysteine proteinases from Caricac and amarcensis. Phytomedicine. 2008;15:237-244.

Seigler DS, Pauli GF, Nahrstedt A, Leen R. Cyanogenicallosides and glucosides from Passiflora edulis and Carica papaya. Phytochemistry. 2002;60:873-882.

Airaodion AI, Ogbuagu EO, Ekenjoku JA, Ogbuagu U, Okoroukwu VN. Antidiabetic effect of ethanolic extract of Carica papaya leaves in alloxan-induced diabetic rats. American Journal of Biomedical Science & Research. 2019;5(3):227-234.

Airaodion AI, Airaodion EO, Ekenjoku JA, Ogbuagu EO, Ogbuagu U. Antiplasmodial potency of ethanolic leaf extract of Carica papaya against Plasmodium berghei in infected Swiss albino mice. Asian Journal of Medical Principles and Clinical Practice. 2019;2(2):1-8

Huet J, Looze Y, Bartik K, Raussens V, Wintjens R, Boussard P. Structural characterization of the papaya cysteine protinases at low pH. Biochem Biophy Res Commun. 2006;341:620-626.

Taiwo EN. Antibacterial activity and medicinal properties of paw paw (Carica papaya). Global Journal of Environmental Sciences. 2010;9(1,2):81-83.

Dominguez de Maria P, Sinisteraa JB, Tsai SW, Alcantara AR. Biotech Adv. 2006;24: 493-499.

Okeniyi JA, Ogunlesi TA, Oyelami OA, Adeyemi LA. Effectiveness of dried Carica papaya seeds against human intestinal parasitosis: A pilot study. J Med Food. 2007;10:493-499.

Airaodion AI, Ogbuagu EO, Airaodion EO, Ekenjoku JA, Ogbuagu U. Pharmacotherapeutic effect of methanolic extract of Telfairia occidentalis leaves on glycemic and lipidemic indexes of alloxan-induced diabetic rats. International Journal of Bio-Science and Bio-Technology. 2019; 11(8):1-17.

Airaodion AI, Ogbuagu EO, Ekenjoku JA, Ogbuagu U, Airaodion EO. Therapeutic effect of methanolic extract of Telfairia occidentalis leaves against acute ethanol-induced oxidative stress in Wistar rats. International Journal of Bio-Science and Bio-Technology. 2019;11(7):179-189.

Airaodion AI, Ngwogu KO, Ngwogu AC Ekenjoku JA. Investigation of antibacterial activity of Vernonia amygdalina leaf extracts against gram-positive and gram-negative bacteria. International Journal of Bio-Science and Bio-Technology. 2019; 11(11):87-93

Anibijuwono II, Udeze AO. Antimicrobial activity of papaya on some pathogenic

organisms of clinical origin from south-western Nigeria. Ethnobotanical Leaflets 2009;13:850–864.

Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal of Ethnopharmacology. 2001;74:87-91.

Ogunjobi AA, Elizabeth OT. Comparative study of antimicrobial activities of ethanol extracts of the Bark and seed of Garcinia kola and Carica papaya. African Journal of Biomedical Research. 2011;14:147–152.

Ocloo A, Nwokolo NC, Nicholas TKD. Dayie. Phytochemical characterization and comparative efficacies of crude extracts of Carica papaya. International Journal of Drug Res. Tech. 2012;2(5):399–406.

Mahmood AA, Sidik K, Salmah I. Wound healing activity of Carica papaya L. aqueous leaf extract in rats. International Journal of Molecular Medicine and Advance Sciences. 2005;1(4):398–401.

Aruljothi S, Uma C, Sivagurunathan P, Bhuvaneswari M. Investigation on antibacterial activity of Carica papaya leaf extracts against wound infection-causing bacteria. International Journal of Research Studies in Biosciences. 2014;2(11):8-12.

Nirosha N, Mangalanayaki R. Antimicrobial activity of leaves and stem extract of Carica papaya L. International Journal of advances in Pharmacy, Biology and Chemistry. 2013;2(3):2277–4688.

Suresh K, Deepa P, Harisaranraj R, Vaira-Achydhan V. Antimicrobial and phytochemical investigation of the leaves of Carica papaya, Cynodon dactylon (L) Pers., Euphorbia hirta L., Melia azedarach L. and Psidium guajava L. Ethnobotanical Leaflets. 2008;12:1184–1191.

Jigna P, Sumitra C. In-vitro antimicrobial activities of extracts of Launaeaprocumbns Roxb. (Labiateae), Vitis vinifera L. (Vitaceae) and Cyperus rotundus L. (Cypperaceae). African Journal of Biomedical Research. 2006;9(2):89–93.

Ezeifeka GO, Orji MU, Mbata TI, Patrick AO. Antimicrobial activities of Cajanus cajan, Garcinia kola and Xylopia aethiopica on pathogenic microorganisms. Bio-technology. 2004;3(1):41–43.