Gene Identification for Bacteriocin Production by Lactic Acid Bacteria Isolated from Selected Fermented Foods

Main Article Content

I. H. Abdulkarim
S. S. D. Mohammed
A. A. Orukotan


Bacteriocin genes are biosynthetic genes which encodes proteins involved in bacteriocin regulation, self-immunity, transport and modification. This research was aimed at identifying the gene for the synthesis of bacteriocin. Four strains of lactic acid bacteria previously isolated from fermented foods (Nono (N2), Ogi (O3), Dawadawa (D1 and D3) and Wara (W3) were identified using molecular technique and used to produce bacteriocin. The bacteriocin activity was assayed against some test bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella sp and methicillin resistant Staphylococcus aureus) using agar well diffusion method and the bacteriocin genes were identified using BAGEL3. The LAB identified were Lactobacillus fermentum O3, Leuconostoc mesenteroides N2, Weissella cibaria D1 and 2 strains of Lactobacillus plantarum D3 and W3. The entire identified LAB was able to produce bacteriocin. The antimicrobial activity showed varied inhibitory effects of the bacteriocins on the test bacteria. Bacteriocin from isolate O3 showed the highest inhibition zone 16mm on S. aureus. The identified gene for these bacteriocins were plnJK gene for Lactobacillus plantarum str WCFS1 and strain LZ95 (W3 and D3), entA gene for Lactobacillus fermentum str 3872 (O3) and ppnC7 gene for Leuconostoc mesenteroides str SRA3 (N2) with the interaction of other peptides were responsible for bacteriocin production.

Bacteriocin, gene identification, lactic acid bacteria, fermented foods, Open Reading Frame (ORF).

Article Details

How to Cite
Abdulkarim, I. H., Mohammed, S. S. D., & Orukotan, A. A. (2020). Gene Identification for Bacteriocin Production by Lactic Acid Bacteria Isolated from Selected Fermented Foods. Asian Journal of Biochemistry, Genetics and Molecular Biology, 3(4), 1-12.
Original Research Article


Zhou F, Zhao H, Bai F, Dziugan P, Liu Y, Zhang B. Purification and characterisation of the bacteriocin produced by Lactobacillus plantarum, isolated from chinese pickle. Czech Journal of Food Science. 2014;32(5).

Udhayashree N, Senbagam D, Senthilkumar B, Nithya K, Gurusamy R. Production of bacteriocin and their application in food products. Asian Pacific Journal of Tropical Biomedicine. 2012;22(1):S406-S410

Karthikeyan V, Santhosh SW. Study of bacteriocin as a food preservative and the L. acidophilus strain as probiotic. Pakistan Journal for Nutrition. 2009;8:335-340.

John GE, Lennox JA. Lactic Acid bacteria profile of some selected food products and their ability to produce bacteriocins. Journal of Advances in Microbiology. 2018;1-8.

Bakkal S, Robinson SM, Riley MA. Bacteriocins of aquatic microorganisms and their potential applications in the seafood industry. In Health and environment in aquaculture. InTech; 2012.

de Jong A, van Hijum SA, Bijlsma JJ, Kok J, Kuipers OP. BAGEL: A web-based bacteriocin genome mining tool. Nucleic Acids Research. 2013;34(Suppl_3):W273 W279.

Mierau I, Kleerebezem M. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Applied Microbiology and Biotechnology. 2005;68(6):705-717.

Todorov SD. Bacteriocins from Lactobacillus plantarum – Production, genetic organization and mode of action: Produção, organização genética e modo de ação. Brazilian Journal of Microbiology. 2009;40(2):209–221.

Ennahar S, Sashihara T, Sonomoto K, Ishizaki A. Class IIa bacteriocins: biosynthesis, structure and activity. FEMS Microbiology Reviews. 2000;24(1):85-106.

Sprules T, Kawulka KE, Vederas JC. NMR solution structure of ImB2, a protein conferring immunity to antimicrobial activity of the type IIa bacteriocin, carnobacteriocin B2. Biochemistry. 2004;43(37):11740-11749.

Smith DR, Lee RW. The mitochondrial and plastid genomes of Volvox carteri: Bloated molecules rich in repetitive DNA. BMC Genomics. 2009;10(1):132.

Halverson KJ, Joseph SC, Scholz MT. U.S. Patent No. 8,991,239. Washington, DC: U.S. Patent and Trademark Office; 2015.

Azadnia PKNA, Khan Nazer AH. Identification of lactic acid bacteria isolated from traditional drinking yoghurt in tribes of Fars province. Iranian Journal of Veterinary Research. 2009;10(3): 235-240

Arimah BD, Ogunlowo OP, Adebayo MA, Jesumirhewe C. Identification of lactic acid bacteria isolated from selected Nigerian foods and comparison of their bacteriocins activities. International Journal of Pharmaceutical and Clinical Research. 2014;6:20-26.

Rajaram G, Manivasagan P, Thilagavathi B, Saravanakumar A. Purification and characterization of a bacteriocin produced by Lactobacillus lactis Isolated from Marine environment. Advanced Journal for Food Science and Technology. 2010;2(2):138-144.

Hena JV, Sudha SS. Immunotoxicological studies of staphylococcin of CA-MRSA. Journal of Advanced Laboratory Research in Biology. 2010;1(2):98-100.

Ogunbanwo ST, Sanni A, Onilude AA. Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lacobacillus brevis OG1. African Journal of Biotechnology. 2003;8:219-27

Sezer G, Güven A. Investigation of bacteriocin production capability of lactic acid bacteria isolated from foods. Lafkas Univesity of Veterinary Fak Derg. 2009;15:45 50.

Salasiah K, Gulam R, Son R, Foo Hooi L. Bacteriocin-producing lactic acid bacteria isolated from traditional fermented food. Malaysian Journal of Medical Sciences. 2001;8:63-68.

Hartnett DJ, Vaughan A, van Sinderen D. Anti-microbial – producing lactic acid bacteria isolated from raw barley and sorghum. Journal of the Institute of Brewing. 2002;108:169-177.

Wang Y, Chen C, Ai L, Zhou F, Zhou Z, Wang L, Guo B. Complete genome sequence of the probiotic Lactobacillus plantarum ST-III. Journal of Bacteriology. 2011;193(1):313-314.

Nissen-Meyer J, Oppegård C, Rogne P, Haugen HS, Kristiansen PE. Structure and mode-of-action of the two-peptide (class-iib) bacteriocins. Probiotics and Antimicrobial Proteins. 2009;1: 38-41.

Nilsen T, Nes IF, Holo H. Enterolysin A, a cell wall-degrading bacteriocin from Enterococcus faecalis LMG 2333. Applied and Environmental Microbiology. 2003;69(5):2975-2984.

Altermann E, Russell WM, Azcarate-Peril MA, Barrangou R, Buck BL, McAuliffe O, Lick S. Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM. Proceedings of the National Academy of Sciences. 2005;102(11):3906-3912

Kim SY, Oh CG, Lee YJ, Choi KH, Shin DS, Lee SK, Lee JH. Sequence analysis of a cryptic plasmid pKW2124 from Weissella cibaria KLC140 and construction of a surface display vector. Journal of Microbiology and Biotechnology. 2013;23(4):545-554.

Nissen-Meyer J, Oppegård C, Rogne P, Haugen HS, Kristiansen PE. Structure and mode-of-action of the two-peptide (class-iib) bacteriocins. Probiotics and Antimicrobial Proteins. 2010;2: 52-60.

Kyriakou PK, Ekblad B, Kristiansen PE, Kaznessis YN. Interactions of a class IIb bacteriocin with a model lipid bilayer, investigated through molecular dynamics simulations. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2016;4:824-835.