Asian Journal of Biochemistry, Genetics and Molecular Biology <p style="text-align: justify;"><strong>Asian Journal of Biochemistry, Genetics&nbsp;and&nbsp;Molecular Biology (ISSN: 2582-3698)</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/AJBGMB/general-guideline-for-authors">Click here for Types of paper</a>). The area of interest of AJBGMB includes but not restricted to all aspects of&nbsp; Biochemistry,&nbsp;Genetics and Molecular Biology. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN peer-reviewed, open access INTERNATIONAL journal.</p> <p>&nbsp;</p> Asian Journal of Biochemistry, Genetics and Molecular Biology en-US Asian Journal of Biochemistry, Genetics and Molecular Biology 2582-3698 A Potential Pathological Role of Wingless Type1 Inducible Signaling Pathway Protein-1 and Betatrophin in Obese Women with Polyststic Ovary Syndrome <p><strong>Background:</strong>&nbsp;Polycystic Ovary Syndrome is a common female endocrinopathy. It is associated with adipokines dysfunctional secretion pattern and insulin resistance, which is considered as the main reason for its clinical feature. Wingless type1 inducible signaling pathway protein-1 is a novel adipokine that displays insulin resistance and adipose tissue inflammation where it strongly related to adipocyte accumulation and regeneration. Betatrophin has a potential role in pancreatic beta-cell proliferation and obesity and several studies showed inconsistent betatrophin levels in patients with diabetes and obesity but, its relation to polycystic ovary syndrome is unclear.</p> <p><strong>Aim:</strong>&nbsp;Investigation of the role of serum wingless type1 inducible signaling pathway protein-1 and betatrophin in normal weight and obese patients with polycystic ovary syndrome. Studying their association with other markers, then determine whether obesity and insulin resistance is associated with them.</p> <p><strong>Methods:</strong>&nbsp;Wingless type1 inducible signaling pathway protein-1 and betatrophin serum levels were measured in 44 patients with polycystic ovary syndrome (22 obese and 22 non-obese) and 44 matched control (22 obese and 22 non-obese) females using specific ELISA kits.&nbsp;</p> <p><strong>Results:</strong>&nbsp;Betatrophin and wingless type1 inducible signaling pathway protein-1 levels were elevated in the polycystic ovary syndrome group (49.4 pg/ml, 187.6 pg/ml) than in the control group (32.08 pg/ml, 108.4 pg/ml) respectively. Moreover, their levels were higher in the obese subgroup than in normal weight subgroup. There were positive correlations between wingless type1 inducible signaling pathway protein-1 and betatrophin in non-obese (r=0.89, p=0.0001***) and in obese (r=0.78, p=0.0001***) polycystic ovary syndrome groups.</p> <p><strong>Conclusion:</strong><em>&nbsp;</em>Betatrophin and wingless type1 inducible signaling pathway protein-1 are associated with adiposity and insulin resistance in polycystic ovary syndrome. Hence wingless type1 inducible signaling pathway protein-1 and betatrophin may play a role in the incidence of polycystic ovary syndrome. They may be valuable in diagnosis and prediction of polycystic ovary syndrome patients.</p> Abrar Gomaa Abd-Elfatah Hassan Mohammed Ali Mohammed Mohammed Doaa Mohammed Mohammed Abd-Elatif Ashraf Taha Abd-Elmouttaleb Mohammed ##submission.copyrightStatement## 2020-03-04 2020-03-04 1 11 10.9734/ajbgmb/2020/v3i330085 Phytochemical Screening and Hepatoprotective Potential of Aqueous Fruit Pulp Extract of Adansonia digitata against CCL4 Induced Liver Damage in Rats <p><strong>Aim: </strong>The current study was carried out to evaluate the hepatoprotective effects of aqueous extract of <em>Adansonia digitata </em>fruit pulp on carbon tetrachloride (CCl<sub>4</sub>) induced liver damage in&nbsp; rats.</p> <p><strong>Place and Duration of Study:</strong> Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Science, between November 2017 and January 2018.</p> <p><strong>Methodology:</strong> <em>A. digitata </em>fruit pulp was extracted by maceration using water; and a concentration of 100 mg/ml was used. Two doses of the aqueous extract (200 mg/kg and 300 mg/kg) and Livoline (25 mg/kg) were used to investigate their hepatoprotective effects on CCl<sub>4</sub>-induced hepatotoxicity in rats.</p> <p><strong>Results:</strong> The two doses of the plant extract showed dose-dependent hepatoprotective effect on CCl<sub>4</sub>-induced hepatotoxicity, as evident by the significant reduction (P&lt;0.05) in serum levels of AST, ALT, ALP and bilirubin along with the improved histopathological liver sections compared to CCl<sub>4</sub>-treated animals.</p> <p><strong>Conclusion:</strong> Due to its hepatoprotective potentials, <em>A. digitata </em>extract may be used to develop standard treatment drugs against some liver disorders when it is further evaluated through extensive researches.</p> A. M. Sa’id A. H. Musa J. A. Mashi F. U. Maigari M. N. Nuhu ##submission.copyrightStatement## 2020-03-07 2020-03-07 12 21 10.9734/ajbgmb/2020/v3i330086 Sex Hormones Levels in Male Welders in Nnewi, South-Eastern Nigeria <p>Welding processes produce toxic fumes consisting of gaseous and aerosol by-products which pose a risk to the male reproductive systems. The rate of infertility has increased globally. This study therefore sought to assess the effects of welding fume inhalation on the sex hormones of welders in Nnewi. A site-by-site cross-sectional study of 45 welders (aged between 18 and 50 years) who were exposed to welding fumes (Test group) and 45 age-matched&nbsp;non-welders (Control group) was carried out. The ages of the Test and Control subjects, as well as the years of exposure of the Test subjects were obtained via questionnaire. A single non-fasting venous blood (about 5 mls) was collected from the ante-cubital space from the subjects via venipuncture between 8:00 AM and 11:00 AM. Serum was separated following clotting, and used for the investigation of the levels of male sex hormones: Testosterone (T), Progesterone (P), Luteinizing Hormone (LH) and Follicle Stimulating Hormone (FSH) among welders. Sex hormones were assayed&nbsp;by Enzyme Linked Immunosorbent Assay. The results showed that Testosterone (2.45±0.34 ng/ml) was significantly lower (p&lt;0.05) in welders when compared to controls (4.94±0.81 ng/ml) and significantly increased (p&lt;0.05) levels of Progesterone (0.54±0.09&nbsp;ng/ml) and LH (7.47±1.56 mIU/L) were found in welders compared with controls Progesterone (0.45±0.08 ng/ml) and LH (5.53±1.05 mIU/L). There was no significant difference in the levels of FSH of the test when compared with the controls. This finding of altered hormone levels indicates a likelihood of reduced reproductive outcome. Exposure to welding fume may therefore interfere with testicular functions leading to disordered reproductive performance, delayed conception, and reduced fertility.</p> C. H. Udeogu I. S. I. Ogbu A. N. Mbachu M. C. Ugwu O. F. Odo O. Ugwu ##submission.copyrightStatement## 2020-03-18 2020-03-18 22 32 10.9734/ajbgmb/2020/v3i330087 Antibacterial Potential of Ethanolic and Aqueous Extracts of Carica papaya Leaves <p><strong>Background: </strong>The search for newer sources of antibiotics is a global challenge pre-occupying research institutions, pharmaceutical companies and academia, since many infectious agents are becoming resistant to synthetic drugs.</p> <p><strong>Aim: </strong>This present study sought to investigate the antibacterial potential of ethanolic and aqueous extracts of <em>Carica papaya</em> leaves.</p> <p><strong>Materials and Methods:</strong> Fresh and healthy leaves of <em>C. papaya </em>were harvested, air dried and milled into powder. The powder was extracted using ethanol and water as solvents. The antibacterial activities of both extracts were determined by diffusion method. Nutrient agar medium was prepared using standard method. Pure cultures of <em>Coliform bacillus</em>, <em>Staphylococcus epidermidis</em>, <em>Streptococcus viridans</em>, <em>Salmonella typhi</em> and <em>Escherichia coli </em>were obtained from the Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture<em>, </em>Abeokuta, Nigeria. The extracts were serially diluted to obtain 1.0%, 0.5%, 0.25% and 0.125% solutions in sterile test tubes. Sterilized 9 mm filter paper disc soaked in the diluted extracts were placed on the plate and incubated for 24 hours at room temperature. The plates were examined for clear zones of inhibition. Presence of zones of inhibition indicated activity.</p> <p><strong>Results:</strong> the results showed that both ethanolic and aqueous extracts of <em>C. papaya</em> leaves exhibit antibacterial activities against <em>C. bacillus, S. epidemidis, S. viridans </em>and<em> E. coli</em> and also inhibited their growth. The effect of the ethanolic extract was greater than that of the aqueous extract. However, this activity was not observed with <em>S. typhi</em>.</p> <p><strong>Conclusion: </strong>The result of the present study showed that <em>C. papaya</em> leaves might effectively inhibit the growth of <em>C. bacillus, S. epidemidis, S. viridans </em>and<em> E. coli</em> but not that of <em>S. typhi</em>. However, the ethanolic extract is more potent than the aqueous extract.</p> Augustine I. Airaodion John A. Ekenjoku Ime U. Akaninyene Anthony U. Megwas ##submission.copyrightStatement## 2020-03-26 2020-03-26 33 38 10.9734/ajbgmb/2020/v3i330088