A Comprehensive Analysis of the Microarray Gene Expression Protocol and Results Using the Mouse T-cell Gene Expression Profile
Onoriode Oyiborhoro *
Department of Biochemistry, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Nigeria.
Oriakhi Kelly
Department of Medical Biochemistry, School of Basic Medical Sciences, University of Benin, Benin City, Edo State, Nigeria.
Esosa S. Uhunmwangho
Department of Biochemistry, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Nigeria.
Kingsley A. Iteire
Department of Anatomy, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Nigeria.
Enoh F. Akpojotor
Department of Computer Engineering, School of Engineering, Delta State Polytechnic, Otefe, Oghara, Delta State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The microarray technology is a very powerful technology that combines molecular biology and computer technology to analyze the gene expression levels for most or all of the genes in a whole genome simultaneously, at very high resolutions. This technology has wide applications, including gene interaction studies for discovery of genes responsible for different diseases; classification of cancers and other diseases; prediction of clinical outcomes or prognosis for different diseases; response to therapy and development of new therapeutic agents, including gene therapy. It is therefore a very potent, unbiased and sensitive technology for the discovery of novel genes involved in the pathogenesis or control of diseases including cancers and autoimmune diseases. In the present study, we seek to give a clear and detailed account of the microarray gene expression protocol using the mouse T-cell gene expression profile, including challenges involved and how to overcome them, as well as detailed analysis of results obtained.
Keywords: cDNA, GeneChip microarray, single stranded oligonucleotide.