Effect of Silymarin on Cathepsin Activity and Oxidative Stress in TNBS-induced Colitis in Rats
Gokhan Bayramoglu
Department of Occupational Health and Safety, Faculty of Health Sciences, Artvin Coruh University, Artvin, Turkey.
Hakan Senturk
Department of Biology, Faculty of Arts and Science, Eskisehir Osmangazi University, Eskisehir, Turkey.
Gungor Kanbak
Department of Biochemistry, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey.
Mediha Canbek
Department of Molecular Biology, Faculty of Arts and Science, Eskisehir Osmangazi University, Eskisehir, Turkey.
Aysegul Bayramoglu
Department of Nutrition and Dietetics, Faculty of Health Sciences, Artvin Coruh University, Artvin, Turkey.
Eda Dokumacioglu *
Department of Nutrition and Dietetics, Faculty of Health Sciences, Artvin Coruh University, Artvin, Turkey.
Selin Engur
Department of Biology, Faculty of Arts and Science, Eskisehir Osmangazi University, Eskisehir, Turkey.
*Author to whom correspondence should be addressed.
Abstract
The purpose of the this study was to defined protective effects of silymarin on the experimental colitis induced by intra-colonic application of 2,4,6-trinitrobenzene sulfonic acid (TNBS). The twenty eight Sprague-Dawley rats were randomly seperated into four groups, each group containing seven rats represent as follows: group 1 determined as control; group 2 determined as colitis-untreated; group 3 determined as colitis rats administered silymarin (50 mg/kg) and group 4 determined as colitis rats administered silymarin (100 mg/kg). Doses of 50 mg/kg and 100 mg/kg silymarin decreased tissue levels of malondialdehyde (MDA), cathepsin L and cathepsin B and activity of myleperoxidase (MPO) enzyme with respect to the colitis group (p<0.05). Based on the results of the study, it can be said that silymarin can be used as an effective treatment agent in inflammatory bowel diseases.
Keywords: Cathepsin, colitis, myleperoxidase, oxidative stress, silymarin.