Variations in Fat-mass and Obesity-associated (FTO) Genes and Allelic Distribution in Some Selected Ethnic Populations in Niger Delta, Nigeria
Asian Journal of Biochemistry, Genetics and Molecular Biology, Volume 13, Issue 2,
Page 22-36
DOI:
10.9734/ajbgmb/2023/v13i2290
Abstract
Aim: To evaluate variations in FTO genes and allelic distribution in some ethnic populations in Niger Delta, Nigeria.
Study Design: Case-controlled observational study.
Place and Duration of Study: Federal Medical Centre, Asaba, Delta State and Safety Molecular Pathology Laboratory, Enugu, Nigeria, between March 2020 and February 2022.
Methodology: The association between sixteen (16) Single Nucleotide Polymorphisms in the FTO gene and some biomarkers of obesity and type 2 diabetes subjects (78 cases and 20 controls) from four different tribes in the Niger Delta region, Nigeria. Multistage sampling method was employed in the subject selection. The subjects were first separated into two groups – new cases (less than a year of diagnosis as Diabetic) and old cases (one year & above). Equal number of samples was then randomly collected from each of the cluster groups. Ten millilitres of blood were collected into EDTA for genotyping using Illumina NextSeq 2000 sequencing platform. Hardy-Weinberg Equilibrium statistical test was used to determine the variation in distribution of the alleles and genotypes within the study population while allelic frequencies were calculated by gene counting. Chi-square (and fisher’s test where chi-square was not applicable) and Odd Ratio (OR) were performed to determine the significant differences and associated risks respectively of the allelic and genotypic frequencies of Type 2 diabetic (T2D) and non-diabetic subjects of the FTO gene variants.
Results: The results of Hardy-Weinberg Statistical Test, Genotype and Allelic Distribution of FTO gene Variants in Obese/T2D Subjects in Different Tribes of Niger Delta are presented in Tables(1-4) for rs73609956 (C>T), rs116753298 (T>C), rs201041270 (A>G), rs531215275 (A>C), rs146056278 (C>T), rs1410999299 (G>A), rs79206939 (A>G), rs145884431(G>A), rs61743972 (G>A), rs201496428 (C>T), rs146138389 (T>C), rs886052102 (A>G), rs144743617 (G>A), rs886052103 (T>A), rs9939609 (A>T) and rs8050136 (A>C). However, no significant differences in analyzed genotype frequencies were found between T2D and healthy controls.
Conclusion: Knowledge of the dominant SNPs in some ethnic groups, may provide platform to delay its expression through informed wise choice of lifestyle change and proper dieting.
- Fat-mass and obesity-associated gene (FTO) variants
- allelic distribution
- ethnic populations
- Niger Delta
- Nigeria
How to Cite
References
Merkestein M, Laber S, McMurray F, Andrew D, Sachse G, Sanderson J, et al. FTO influences adipogenesis by regulating mitotic clonal expansion. Nat Commun. 2015;6:6792.
Smemo S, Tena JJ, Kim KH, Gamazon ER, Sakabe NJ, Gómez-Marín C, et al. Obesity-associated variants within FTO form long-range functional connections with IRX3. Nature. 2014;507(7492):371-5.
Claussnitzer M, Dankel SN, Kim KH, Quon G, Meuleman W, Haugen C, et al. FTO obesity variant circuitry and adipocyte browning in humans. N Engl J Med. 2015;373(10):895-907.
Kim YJ, Lee HS, Kim YK, Park S, Kim JM, Yun JH, et al. Association of metabolites with obesity and Type 2 diabetes based on FTO genotype. PLOS ONE. 2016; 11(6):e0156612.
Shahid A, Rana S, Saeed S, Imran M, Afzal N, Mahmood S. Common variant of FTO gene, rs9939609, and obesity in Pakistani females. BioMed Res Int. 2013;9:1-7.
Han L, Tang L, Wang C, Chen Z, Zhang T, Chen S, et al. Fat mass and obesity-associated gene rs11642015 polymorphism is significantly associated with prediabetes and type 2 diabetes subsequent to adjustment for body mass index. Biomed Rep. 2014;2(5):681-6.
Peng S, Zhu Y, Xu F, Ren X, Li X, Lai M. FTO gene polymorphisms and obesity risk: a meta-analysis. BMC Med. 2011;9:71.
Meyre D, Delplanque J, Chèvre JC, Lecoeur C, Lobbens S, Gallina S, et al. Genome-wide association study for early-onset and morbid adult obesity identifies three new risk loci in European populations. Nat Genet. 2009;41(2):157-9.
Locke AE, Kahali B, Berndt SI, Justice AE, Pers TH, Day FR, et al. Genetic studies of body mass index yield new insights for obesity biology. Nature. 2015;518 (7538):197-206.
Frayling TM, Timpson NJ, Weedon MN, Zeggini E, Freathy RM, Lindgren CM, et al. A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity. Science. 2007;316(5826):889-94.
Zeggini E, Weedon MN, Lindgren CM, Frayling TM, Elliott KS, Lango H, et al. Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes. Science. 2007;316 (5829):1336-41.
Kim YJ, Lee HS, Kim YK, Park S, Kim JM, Yun JH, et al. Association of metabolites with obesity and Type 2 diabetes based on FTO genotype. Plos One. 2016;11(6):e0156612.
Liu Y, Liu Z, Song Y, Zhou D, Zhang D, Zhao T, Yang Y, Feng G, Li J, Zhang J, Liu S, Zhang Z, He L, Xu H. Meta-analysis added power to identify variants in FTO associated with type 2 diabetes and obesity in the Asian population. Obesity. 2010;18(8):1619-24.
Nnaemeka AN. Environmental pollution and associated health hazards to host communities (case study: Niger Delta region of Nigeria). Cent Asian J Environ Sci Technol Innov. 2020;1(1):30-42.
Adeyemo A, Chen G, Zhou J, Shriner D, Doumatey A, Huang H et al. FTO genetic variation and association with obesity in West Africans and African Americans. Diabetes. 2010;59(6):1549-54.
Hennig BJ, Fulford AJ, Sirugo G, RaycoSolon P, Hattersley AT, Frayling TM et al. FTO gene variation and measures of body mass in an African population. BioMed Cent Med Genet. 2009;10(21):1-8.
Adeyemo A, Chen G, Zhou J, Shriner D, Doumatey A, Huang H, et al. FTO genetic variation and association with obesity in West Africans and African Americans. Diabetes. 2010;59(6):1549-54.
Apalasamy YD, Ming MF, Rampal S, Bulgiba A, Mohamed Z. Genetic association of SNPs in the FTO gene and predisposition to obesity in Malaysian Malays. Braz J Med Biol Res. 2012;45 (12):1119-26.
Yang Y, Liu B, Xia W, Yan J, Liu HY, Hu L, et al. FTO genotype and T2 diabetes mellitus: spatial analysis and meta-analysis of 62 case-control studies from different regions. Genes. 2017;8(2): 70-87.
Speliotes EK, Willer CJ, Berndt SI, Monda KL, Thorleifsson G, Jackson AU, et al. Association analyses of 249,796 individuals reveal 18 new loci associated with body mass index. Nat Genet. 2010;42(11):937-48.
Li H, Kilpeläinen TO, Liu C, Zhu J, Liu Y, Hu C, et al. Association of genetic variation in FTO with risk of obesity and type 2 diabetes with data from 96,551 East and South Asians. Diabetologia. 2012;55(4): 981-95.
Peng S, Zhu Y, Xu F, Ren X, Li X, Lai M. FTO gene polymorphisms and obesity risk: A meta-analysis. BMC Med. 2011; 9:71.
Bressler J, Kao WHL, Pankow JS, Boerwinkle E. Risk of Type 2 diabetes and obesity is differentially associated with variation in FTO in whites and African-Americans in the ARIC study. Plos One. 2010;5(5):e10521.
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