Protective and Curative Potential of Ethanol Leaf Extract of Corchorus olitorius against Potassium Bromate-Induced Renal Toxicity
Asian Journal of Biochemistry, Genetics and Molecular Biology, Volume 13, Issue 2,
Aim: The objective of this study was to assess the protective and curative potential of ethanol leaf extract of Corchorus olitorius against potassium bromate (KBrO3)-induced renal toxicity.
Methodology: Corchorus olitorius was extracted using a soxhlet extractor and ethanol as the solvent. After becoming accustomed to the lab, 24 mature male Wistar rats were randomly assigned to groups A, B, C, and D. Group A received oral distilled water as treatment. Animals in groups B, C, and D got 100 mg/kg body weight of potassium bromate while groups C and D also received 100 and 200 mg/kg body weight of Corchorus olitorius respectively. Fresh potassium bromate and groups C and D extract were administered to rats every day by oral gavage. After taking the drug for the recommended 28 days, blood and kidney samples were collected. Renal biomarkers were evaluated using conventional methods.
Results: Significant (P0.05) increase in the serum concentrations of creatinine, urea, uric acid, sodium (Na+), potassium (K+), chloride (Cl–), and bicarbonate (HCO3–) were observed following potassium bromate administration in comparison to the control group. KBrO3 poisoning also increased the levels of the inflammatory proteins interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-) in the kidneys compared to the control group. Yet when KBrO3 and C. olitorius leaf extract were administered together, levels of all kidney indicators were significantly reduced in a dosage-dependent manner, with 200 mg/kg being the most efficient dose.
Conclusion: This study found that C. olitorius leaf extract, particularly at the higher dose of 200 mg/kg, was successful in reducing a number of the parameters examined that had been negatively impacted by KBrO3. It may be advantageous to include C. olitorius leaf in edible products that may contain KBrO3, such as flour, bread, or cakes, as it is a well-known dietary prebiotic with established safety profiles in humans. Further research is required to determine whether C. olitorius leaves can reduce the toxicity of KBrO3 in human organs and other animal strains, as well as perhaps treat it.
- Corchorus olitorius
- potassium bromate
- renal toxicity
How to Cite
Environmental Protection Agency. Toxicological review of bromate. Washington, DC, USA: Environmental Protection Agency. 2021;(CAS No. 15541-45-4).
Oloyede OB, Sunmonu TO. Potassium bromate content of selected bread samples in Ilorin, central nigeria and its effect on some enzymes of rat liver and kidney. Food Chem Toxicol. 2019;47:2067-2070.
Airaodion AI, Ewa O, Ogbuagu EO, Ogbuagu U, Agunbiade AP, Oloruntoba
AP. Evaluation of potassium bromate in bread in Ibadan metropolis: Fifteen years after ban in Nigeria. Asian Food Science Journal. 2019;7(4):1–7.
Airaodion AI, Awosanya OO, Ogbuagu EO, Ogbuagu U, Njoku OC, Esonu C, Airaodion EO. Assessment of bread in ogbomoso metropolis for the presence of potassium bromate. Asian Journal of Research in Biochemistry. 2019;4(2):1-6.
Fisher N, Hutchinson JB, Berry R, Hardy J, Ginocchio AV, Waite V. Long-term toxicity and carcinogenicity studies of the bread improver potassium bromate 1. Studies in rats. Food Cosmet Toxicol. 1979;17:33-39.
Ugwu CN, Abali IO, Iwuoha CE, Chika-Igwenyi NM, Onyeaghala CA, Orji SF, Igwenyi C, Uche CL, Onyekachi OIN, Nwobodo MU, Airaodion AI. Ameliorative effect of Parkia biglobosa (African locust bean) seed against potassium bromate-induced oxidative stress. Merit Research Journal of Medicine and Medical Sciences. 2022;10(8):213-219.
Onyekachi OIN, Orji SF, Ugwu CN, Igwenyi C, Uche CL, Abali IO, Nwobodo MU, Iwuoha CE, Chika-Igwenyi NM, Onyeaghala CA, Agu FU, Airaodion AI. Hepatocellular injury ameliorated by a common african food, Parkia biglobosa, Asian Journal of Research in Medical and Pharmaceutical Sciences. 2022;11(4):26-34.
Abali IO, Chika-Igwenyi NM, Agu, FU, Onyeaghala CA, Orji SF, Ugwu CN, Igwenyi C, Uche CL, Onyekachi OIN, Nwobodo MU, Iwuoha CE, Airaodion AI. Nephro-protective efficacy of african locust bean seed against potassium bromate-induced renal damage. Asian Journal of Biochemistry, Genetics and Molecular Biology. 2022;12(3):28-36.
Ezirim EO, Uche CL, Abali IO, Iwuoha CE, Chika-Igwenyi NM, Onyeaghala CA, Orji SF, Ugwu CN, Ugwu NI, Igwenyi C, Airaodion AI. Therapeutic potential of Parkia biglobosa seed against potassium bromate-induced testicular toxicity. International Journal of Research and Reports in Gynaecology. 2022;5(3):78-89.
Ugwu CN, Iwuoha CE, Chika-Igwenyi NM, Onyeaghala CA, Orji SF, Igwenyi C, Uche CL, Onyekachi OIN, Nwobodo MU, Abali IO, Airaodion AI. Chemotherapeutic propensity of Africa locust bean (Parkia biglobosa) seed on lipid profile against potassium bromate-induced cardiotoxicity. Journal of Applied Life Sciences International. 2022;25(8):24- 31.
Ezirim EO, Onyeaghala CA, Orji SF, Ugwu CN, Igwenyi C, Uche CL, Abali IO, Onyekachi OIN, Nwobodo MU, Iwuoha CE, Chika-Igwenyi NM, Airaodion AI. Attenuation of potassium bromate-induced infertility by African locust bean (Parkia biglobosa) seed. Asian Journal of Medicine and Health. 2022;20(5):12-23.
Iwuoha CE, Ezirim EO, Onyeaghala CA, Orji SF, Ugwu CN, Igwenyi C, Uche CL, Abali IO, Onyekachi OIN, Nwobodo MU, Agu FU, Chika-Igwenyi NM, Airaodion AI. Perturbation of sex hormones by potassium bromate and preventive effect of African locust bean (Parkia biglobosa) seed. Asian Journal Research in Biochemistry. 2022;10(5):22-31.
Ugwu NI, Uche CL, Ogbenna AA, Okite UP, Chikezie K, Ejikem PI, Ugwu CN, Otuka OAI, Ezirim EO, Onyekachi OIN, Nwobodo MU, Abali IO, Iwuoha CE, AI Airaodion. Blood Coagulation Normalization Effect of Parkia biglobosa Seed on Potassium Bromate-induced Coagulopathy. West African Journal of Medicine. 2023;40(2):148-156.
Spassova MA, Miller DJ, Nikolov AS. Kinetic modeling reveals the roles of reactive oxygen species scavenging and DNA repair processes in shaping the dose-response curve of KBrO₃-induced DNA damage. Oxid Med Cell Longev. 2015;2015: 764375.
Ali BH, Za’abi MA, Karaca T, Suleimani YA, Balushi KA, Manoj P, Ashique M, Nemmar A. Potassium bromate-induced kidney damage in rats and the effect of gum acacia thereon. Am J Transl Res. 2018;10(1):126-137.
Airaodion AI, Ogbuagu EO, Ogbuagu U, Awosanya OO, Airaodion EO. Effect of Methanolic extract of Corchorus olitorius leaves on hypoglycemic and hypolipidaemic activities in albino rats. Asian Plant Research Journal. 2019;2(4):1-13.
Adebo HO, Ahoton LE, Quenum FJB, Adoukonou-Sagbadja H, Bello DO, Chrysostome AM. Ethnobotanical knowledge of jute (Corchorus olitorius L.) in Benin. Eur J Med Plant. 2018;26(1):1–11.
Khan MSY, Bano S, Javed K, Mueed MA. A comprehensive review on the chemistry and pharmacology of Corchorus species – A source of cardiac glycosides, triterpenoids, ionones, flavonoids, coumarins, steroids and some other compounds. J Sci Ind Res. 2006;65:283–298.
Ugwu CN, Igwenyi C, Uche CL, Abali IO, Onyekachi OIN, Nwobodo MU. In vivo attenuation of experimentally-induced oxidative stress by common African vegetable (Corchorus olitorius). GSC Biological and Pharmaceutical Sciences. 2022;21(01):116-122.
Ugwu CN, Abali IO, Agu FU, Aguh KJ, Onyekachi OIN, Nwobodo MU, Chika-Igwenyi NM, Onyeaghala CA, Uche CL, Orji SF, Igwenyi C, Airaodion AI. Prophylactic Potential of Corchorus olitorius Leaves against Experimentally-induced Dyslipidemia. Asian Journal of Cardiology Research. 2022;7(4):6-13.
Aguh KJ, Onyekachi OIN, Nwobodo MU, Chika-Igwenyi NM, Onyeaghala CA, Agu FU. Hepatoprotective potential of Corchorus olitorius leaves against potassium bromate-induced hepatotoxicity. Merit Research Journal of Microbiology and Biological Sciences. 2022; 10(5):046-054.
Airaodion AI, Ogbuagu EO, Ewa O, Ogbuagu U, Awosanya OO, Adekale OA. Ameliorative Efficacy of Methanolic Extract of Corchorus olitorius Leaves against Acute Ethanol-Induced Oxidative Stress in Wistar Rats. Asian Journal of Biochemistry, Genetics and Molecular Biology. 2019;7(6):1-9.
Airaodion AI, Uche CL, Ezirim EO, Otuka OAI, Abali OI, Nwobodo MU, Ejikem PI, Okite UP, Chikezie K. Mitigation of experimentally-induced testicular toxicity by Corchorus olitorius leaves. EC Pharmacology and Toxicity. 2023;11(1):14-24.
Onyeaghala CA, Nwobodo MU, Ezirim EO, Chika-Igwenyi NM, Agu FU, Aguh KJ, Orji SF, Igwenyi C, Otuka OAI, Ugwu CN, Uche CL, Abali IO, Onyekachi OIN, Airaodion AI. Common African Vegetable (Corchorus olitorius) Alleviated Potassium Bromate-Induced Sperm Abnormalities. EC Gynaecology. 2022;11(11): 09-19.
Ezirim EO, Ugwu CN, Uche CL, Abali IO, Onyekachi OIN, Nwobodo MU, Chika-Igwenyi NM, Onyeaghala CA, Aguh KJ, Agu FU, Orji SF, Igwenyi C, Airaodion AI. Effect of Corchorus olitorius leaves on sex hormones of animals induced with potassium bromate. International Journal of Chemical & Life Science. 2022;11(10): 22-31.
Khan RA, Khan MR, Sahreen S. Protective effects of rutin against potassium bromate induced nephrotoxicity in rats. BMC Complement Altern Med. 2012;12: 204.
Ahmad MK, Naqshbandi A, Fareed M, Mahmood R. Oral administration of a nephrotoxic dose of KBrO3, a food additive alters renal redox and metabolic status and inhibits brush border membrane enzymes in rats. Food Chem. 2012;134: 980-985.
Airaodion AI, Ngwogu AC, Ekenjoku JA, Ngwogu KO. Hepatoprotective potency of ethanolic extract of Garcinia kola (Heckel) seed against acute ethanol-induced oxidative stress in Wistar rats. International Research Journal of Gastroenterology and Hepatology. 2020;3(2):1-10.
Airaodion AI, Ogbuagu U, Ekenjoku JA, Ogbuagu EO, Airaodion EO, Okoroukwu VN. Hepato-protective efficiency of ethanol leaf extract of Moringa oleifera against hydrocarbon exposure. International Journal of advances in Herbal and Alternative Medicine. 2019;03(01):32-41.
National Research Council. Guide for the Care and Use of Laboratory Animals, 8th ed. The National Academies Press: Washington, DC, USA; 2011.
Mohamed EAK, Saddek EA. The protective effect of taurine and/or vanillin against renal, testicular, and hematological alterations induced by potassium bromate toxicity in rats. The Journal of Basic and Applied Zoology. 2019;80(3):1-11.
Topcu-Tarladacalisir Y, Sapmaz-Metin M, Karaca T. Curcumin counteracts cisplatin-induced nephrotoxicity by preventing renal tubular cell apoptosis. Ren Fail. 2016;38:1741-1748.
Ben Saad H, Driss D, EllouzChaabouni S, Boudawara T, Zeghal KM, Hakim A, Ben Amara I. Vanillin mitigates potassium bromate-induced molecular, biochemical and histopathological changes in the kidney of adult mice. Chem Biol Interact. 2016;25:102-113.
Dodd DE, Layko DK, Cantwell KE, Willson GA, Thomas RS. Subchronic toxicity evaluation of potassium bromate in Fischer 344 rats. Environ Toxicol Pharmacol. 2013;36:1227-1234.
Nishioka H, Fujii H, Sun B, Aruoma OI. Comparative effcacy of oligonol, catechin and (-)-epigallocatechin 3-O-gallate in modulating the potassium bromate-induced renal toxicity in rats. Toxicology. 2006;226:181-187.
Abd-Allah AR, Al-Majed AA, Mostafa AM, AlShabanah OA, Din AG, Nagi MN. Protective effect of arabic gum against cardiotoxicity induced by doxorubicin in mice: a possible mechanism of protection. J Biochem Mol Toxicol. 2002;16:254- 259.
Ahmad MK, Khan AA, Mahmood R. Taurine ameliorates potassium bromate-induced kidney damage in rats. Amino Acids. 2013;45:1109-1121.
Airaodion AI, Alabi OJ, Ogbuagu EO, Atiba FA, Olawoyin DS. Nephro and hepatotoxic effect of air-freshener in Wistar rats. Asian Journal of Research in Nephrology. 2020;3(2):1-9.
Airaodion AI, Megwas AU, Ekenjoku JA, Ngwogu KO, Ngwogu AC. Nephro- and hepato-toxicity of common household insecticides used in Nigeria. International Research Journal of Gastroenterology and Hepatology. 2020;3(2):21-28.
Akomolafe SF, Olasehinde TA, Adewale OO. Curcumin improves biomolecules associated with renal function and attenuates oxidative injury and histopathological changes in potassium induced toxicity in rats’ kidneys. Biol Trace Elem Res. 2021;199:197-204.
Abd-Elmaksoud HA, Abdel-Hamid OM, Desouki, A. Biochemical evaluation of parsley with or without alfacalcidol on treatment of renal dysfunction experimentally induced by potassium bromate. ARC J Nutr Growth, 2020;6: 27-35.
Kanadi MA, Wudil AM, Alhassan AJ. Dose-dependent chemopreventive effect of methanol extract of Carica papaya seed on potassium bromate-induced nephrotoxicity in rats. Asian J Biochem Genet Mol Biol. 2019;2:1-12.
Dajas F, Arrendondo F, Echeverry C, Ferreira M, Morquio A and Rivera F. Flavonoids and the brain. Evidences and mechanisms for a protective capacity. Curr. Neuro. Pharma. 2005;3(3):1-2.
Calleja DO. Saluyot now a popular vegetable worldwide. Inquirer; 2011.
Mahbubul Islam. Biochemistry, Medicinal and Food values of Jute (Corchorus capsularis L. and C. olitorius L.) leaf: A Review. International Journal of Enhanced Research in Science Technology & Engineering, ISSN: 2319-7463, November. 2013;2(11):35-44.
Consolacion YR, Vivar JLA, Tan MCS, Chien-Chang S. Chemical Constituents of Corchorus olitorius L. International Journal of Pharmacognosy and Phytochemical Research. 2016;8(12): 2085-2089.
Anup KD, Bag S, Sahu R, Dua TK, Sinha MK, Gangopadhyay M, Zaman K, Dewanjee S. Protective effect of Corchorus olitorius leaves on sodium arsenite-induced toxicity in experimental rats. Journal of food and chemical Toxicology. 2010;48:326-335.
Sule OJ, Arhoghro EM, Erigbali P. protective and curative activity of ethanol leaf extract of Corchorus olitorius in thioacetamide exposed rats. World Journal of Pharmaceutical Research, 2017; 6(12):25-36.
Ogbuagu EO, Airaodion AI, Ogbuagu U, Nweke IN, Unekwe PC. Nephrotoxicity of ethanol extract of Xylopia aethiopica fruit in Wistar rats. International Journal of Advances in Nephrology Research. 2021;4(1):1-16.
Adewale OO, Aremu KH, Adeyemo AT. Assessment of combined toxic effects of potassium bromate and sodium nitrite in some key renal markers in male Wistar rats. Res J Heal Sci. 2020;8(1):6-17.
Stenvinkel P, Ketteler M, Johnson RJ. IL-10, IL-6, and TNF-α: central factors in the altered cytokine network of uremia‒the good, the bad, and the ugly. Kidney Int. 2015;67(4):1216–1233.
Elsayed M, Barakat H. Vitamins C and E alleviate nephrontoxicity-induced by potassium bromate in rats. J Appl Life Sci Int. 2016;8:1–8.
Okoko T. Bromate causes significant cytotoxicity and modulation of the inflammatory response. Asian J Biotechnol Genet Eng. 2020;3(4):1-8.
Bayomy NA, Soliman GM, Abdelaziz EZ. Effect of potassium bromate on the liver of adult male albino rat and a possible protective role of vitamin C: histological, immunohistochemical, and biochemical study. Anat Rec (Hoboken). 2016; 299(9):1256-1269.
Ali BH, Za'abi MA, Karaca T. Potassium bromate induced kidney damage in rats and the effect of gum acacia thereon. Am J Transl Res. 2018;1:126-137.
Edeogu CO, Kalu ME, Famurewa AC. Nephroprotective effect of Moringa oleifera seed oil on gentamicin-induced nephrotoxicity in rats: biochemical evaluation of antioxidant, anti-inflammatory, and antiapoptotic pathways. J Am Coll Nutr. 2021;39(4): 307-315.
Abdel-Daim MM, Khalil SR, Awad A. Ethanolic extract of Moringa oleifera leaves influences NF-κB signaling pathway to restore kidney tissue from cobalt-mediated oxidative injury and inflammation in rats. Nutrients. 2020;12(4):1031. DOI: doi: 10.3390/nu12041031).
Luetragoon T, Sranujit RP, Noysang C. Bioactive compounds in Moringa oleifera Lam. Leaves inhibit the pro inflammatory mediators in lipopolysaccharide-induced human monocyte derived macrophages. Molecules. 2020;25(1):191. DOI: 10.3390/molecules25010191
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