Asian Journal of Biochemistry, Genetics and Molecular Biology

  • About
    • About the Journal
    • Submissions & Author Guideline
    • Accepted Papers
    • Editorial Policy
    • Editorial Board Members
    • Reviewers
    • Propose a Special Issue
    • Printed Hard copy
    • Subscription
    • Membership
    • Publication Ethics and Malpractice Statement
    • Digital Archiving
    • Contact
  • Archives
  • Indexing
  • Publication Charge
  • Submission
  • Testimonials
  • Announcements
Advanced Search
  1. Home
  2. Archives
  3. 2018 - Volume 1 [Issue 4]
  4. Original Research Article

Submit Manuscript


Subscription



  • Home Page
  • Author Guidelines
  • Editorial Board Member
  • Editorial Policy
  • Propose a Special Issue
  • Membership

Total Phenolics, Flavonoids Contents, Antioxidant Activity and DNA Protective Effect of Lenten Rose (Helleborus orientalis)

  • Meral Öztürk
  • İbrahim Bulduk
  • Safiye Elif Korcan
  • Recep Liman
  • Funda Karabağ Çoban
  • Mustafa Kargıoğlu
  • Muhsin Konuk

Asian Journal of Biochemistry, Genetics and Molecular Biology, Page 1-12
DOI: 10.9734/ajbgmb/2018/v1i430047
Published: 12 March 2019

  • View Article
  • Download
  • Cite
  • Statistics
  • Share

Abstract


The aims of the present study were to evaluate the antioxidant activity and DNA Protective effect of Helleborus orientalis (HO) leaf extract against oxidative damage, and to determine the total phenolic and flavonoid contents of the plant species studied.


Methods: The total phenol content (TPC) of H. orientalis (Ranunculaceae) extract was determined using the Folin-Ciocalteu technique. The aluminum chloride colorimetric assay in the determination of The total flavonoid content (TFC) and was used, Analysis of Phenolic Acids was identified by High-Performance Liquid Chromatography (HPLC). Antioxidant activity was analyzed by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Assay. Protective effect of H. orientalis leaf extract against to H2O2 was evaluated by using TAS, TOS methods and Comet assay.


Key Findings: H. orientalis ethanol extracts contain high amounts of antioxidants. The HO leaf methanol extract (LME) decreased the DNA damage at all tested concentrations in a dose-dependent manner (r=0.86 p<0.01) against to H2O2.


Conclusions: The total phenol content in the extracts of different parts of the plant varied from 4.00 mg GAE/1 gr to 19.42 mg GAE/1 gr. The flowers had the highest phenol content (19.42 mg GAE/1 gr sample) and followed by the leaves (17.20 mg GAE/1 gr sample). The total flavonoid content in the extracts from different parts of the plant varied from 2.57 mg QE/1 gr to 11.88 mg QE/1 gr. The flowers had the highest flavonoid content (11.88 mg QE/1 gr sample) and followed by the leaves (10.21 mg QE/1 gr sample).


Antioxidant activity of fractions was explained as a percentage of DPPH radicals’ scavenging and IC50 values (μg/ml). Leaf and flowers of HO are richer in antioxidant than its root and stem. As the concentration of leaf extracts used increased, the DNA protective effect increased and it was statistically significant at overdoses of 2500 μg/mL. Total antioxidant status (TAS) levels were significantly (p <0.05) decreased in the H2O2 group (3.4±0.21) but H. orientalis was significantly (p<0.05) increased TAS levels in this group. When the concentration of leaf extracts used increased, the protective effect has also increased and statistically significant at overdoses of 2500 μg / mL (6.3±0.67). Total oxidant status (TOS) levels were significantly (p <0.05) increased in the H2O2 group (25.3±0.74) and H. orientalis was significantly (p<0.05) decreased TOS levels in groups.


Keywords:
  • Antioxidant activity
  • flavonoids
  • Helleborus orientalis
  • oxidative DNA damage
  • phenolics
  • protective effect.
  • Full Article - PDF
  • Review History

How to Cite

Öztürk, M., Bulduk, İbrahim, Korcan, S., Liman, R., Çoban, F., Kargıoğlu, M., & Konuk, M. (2019). Total Phenolics, Flavonoids Contents, Antioxidant Activity and DNA Protective Effect of Lenten Rose (Helleborus orientalis). Asian Journal of Biochemistry, Genetics and Molecular Biology, 1(4), 1-12. https://doi.org/10.9734/ajbgmb/2018/v1i430047
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
  • Abstract View: 2085 times
    PDF Download: 1182 times

Download Statistics

Downloads

Download data is not yet available.
  • Linkedin
  • Twitter
  • Facebook
  • WhatsApp
  • Telegram
Make a Submission / Login
Information
  • For Readers
  • For Authors
  • For Librarians
Current Issue
  • Atom logo
  • RSS2 logo
  • RSS1 logo


© Copyright 2010-Till Date, Asian Journal of Biochemistry, Genetics and Molecular Biology. All rights reserved.