Identification of Fusarium spp. Associated with Potato Tubers in Upper Egypt by Morphological and Molecular Characters
Asian Journal of Biochemistry, Genetics and Molecular Biology,
Potato (Solanum tuberosum L.) is an important crop which holds promise for food security considering the global population growth rate. Fusarium dry rot is one of the most significant diseases of potato. To build up strategies for the control of this disease, it ought to be made primarily a correct diagnosis and identification of the pathogen. A total of 504 Fusarium isolates were recovered from potato tubers collected from Upper Egypt. Fusarium isolates were identified based on morphology and partial DNA sequencing of β-tubulin (TUB) genes. 62.5% of the isolates were identified as F. sambucinum, followed F. oxysporum (57.5%), then F. verticillioides (56.25%) and F. incarnatum (47.5%). All the tested Fusarium species were able to produce amylase. The pathogenicity of the isolates was tested by inoculation of healthy potato tubers; all of the tested isolates were pathogenic to healthy potato tubers. F. sambucinum had a highly virulent effect.
- Potato (Solanum tuberosum L.)
- Fusarium spp.
How to Cite
Mahgoub HAM, Eisa GSA, Youssef MAH. Molecular, biochemical and anatomical analysis of some potato (Solanum tuberosum L.) cultivars growing in Egypt. Genet Eng Biotechnol J. 2015;13:39-49.
Younes NA, El-Sheikh AMM, Aboul-Nasr MH. Heat and freezing pre-thermal treatments as a means of freeing potatoes from mosaic virus and its effects on potato plants quality characters. Journal of Phytopathology and Pest Management. 2014;2:44-52.
Omar GF, Fouad H, Mohamed, Khalid E. Abd El-Hamed, Basma AE. Influence of plant density and genotype on potato minituber production from microshoots and microtubers. Int J Environ Sci Te. 2018; 17(1):77-84.
Abd-Elhak MZ. Potato production and storage in Egypt. Egyptian Ministry of Agriculture: The Horticulture Research Institute. 2005;84(9):62-72.
Kabeil SS, Lashin SM, El- Masry MH, El-Saadani MA, Abd-Elgawad MM, Aboul-Einean AM. Potato brown rot disease in Egypt: Current status and prospects. American-Eurasian J. Agric. &Environ. Sci. 2008;4(1):44-54.
Du M, Ren X, Sun Q, Wang Y, Zhang R. Characterization of Fusarium spp. causing potato dry rot in China and susceptibility evaluation of Chinese potato germplasm to the pathogen. Potato Res. 2012;55:175–184.
Sharma S, Lal M. Dry rot. In Singh BP, Nagesh M, Sharma S, Sagar V, Jeevvlatha A, Sridhar J (eds). A manual on diseases and pest of potato‐technical Bulletin No. 101. ICAR-Central Potato Research Institute, Shimla, HP, India. 2015;17-19.
Leslie JF, Summerell BA. The Fusarium laboratory manual. UK: Blackwell Publishing Ltd. 2006;388.
Wharton PS, Hammerschmidt R, Kirk W.Fusarium dry rot. Michigan State University Extension Bulletin. 2007;29- 95.
Powelson ML, Rowe HC. Managing diseases caused by seedborne and soilborne fungi and fungus-like pathogens. In: Johnson DA (Ed), potato health management. APS Press, St. Paul, MN. 2008;183-195.
Secor GA, Salas B. Fusarium dry rot and Fusarium wilt. In: Compendium of potato diseases, St. Paul, MN, USA: APS Press. 2001;23–25.
El-Hassan KI, El-Saman MG, Mosa AA, Mostafa MH. Variation among Fusarium spp. the causal of potato tuber dry rot in their pathogenicity and mycotoxins production. Egypt. J. Phytopathol. 2007;35: 53–68.
El-Kot GAN. Biological control of black scurf and dry rot of potato. Egypt. J. Phytopathol. 2008;36:45–56.
Hanson LE, Schwager SJ, Loria R. Sensitivity to thiabendazole in Fusarium species associated with dry rot of potato. Phytopathology. 1996;86:378–384.
Kim JC, Lee YW. Sambutoxin, a new mycotoxins produced by toxic Fusarium isolates obtained from rotted potato tubers. Appl Environ Microbiol. 1994;60:4380–4386.
Gachango E, Hanson LE, Rojas A, Hao JJ, Kirk WW. Fusarium spp. causing dry rot of seed potato tubers in Michigan and their sensitivity to fungicides. Plant Dis. 2012; 96:1767–1774.
Gherbawy YA. RAPD profile analysis of isolates belonging to different formae speciales of Fusarium oxysproum. Cytologia. 1999;64:269-276.
O´Donnell K, Gherbawy YA, Schweigkofler W, Adler A, Prillinger H. Comparative phylogenetic analysis of Fusarium oxysporum and related species from maize using DNA sequence and RAPD data. J Phytopathology. 1999;147: 445-452.
Laurence MH, Summerell BA, Liew ECY.Fusarium oxysporum f. sp. canariensis: Evidence for horizontal gene transfer of putative pathogenicity genes. Plant Pathol. 2015;64:1068–1075.
O’Donnell K. Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia. 2000;92(5): 919-938.
Aydin MH, İnal. Comparative susceptibility of some commercial potato cultivars to Fusarium sambucinum and F. solani isolates causing tuber dry rot. Appl Ecol Env Res. 2018;16(4):4879-4892.
Geiser DM, JimenzGasco MM, Kang S, Mk alowska I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O’Donnell K. FUSARIUM-IDv.1.0: A DNA sequence database for identifying Fusarium. Eur J Plant Pathol. 2004;110:473-479.
Nalim FA, Samuels GJ, Wijesundera RL, Geiser DM. New species from the Fusarium solani species complex derived from perithecia and soil in the old world tropics. Mycologia. 2011;103:1302-1330.
Castaño R, Scherm B, Avilés M. Genetic Diversity of Fusarium oxysporum f. sp. dianthi in Southern Spain. J Mycol. 2014; 14.
Taha EM, Rabie W, Mousa ASM, Yasser MM, Fahmy ZM. Phylogenetic diversity among Egyptian isolates of Fusarium species from sugar beet. Int j Agric Res Technol. 2016;12(2):365-381.
Edlind TD, Li J, Visvesvara GS, Vodkin MH, Mclaughlin GL, Katiyar SK. Phylogenetic analysis of β-tubulin sequences from amitochondrial protozoa. Mol Phylogenet Evol. 1996;5: 359–367.
Thon MR, Roys DJ. Partial β-tubulin gene sequences for evolutionary studies in the Basidiomycotina. Mycologia.1999;91(3): 468-474.
Walton JD. Deconstructing the cell wall. Plant Physiol. 1994;104(4):1113-1118.
Albersheim P, Jones TM, English PD. Biochemistry of the cell wall in relation to infective processes. Annu Rev. Phytopathol. 1969;7:171-194.
Mullen JM, Bateman DF. Enzymatic Degradation of Potato Cell Walls in Potato Virus X-Free and Potato Virus X-Infected Potato Tubers by Fusarium roseum (Avenaceum). Phytopathology. 1975;65: 797-802.
Gashgari RM, Gherbawy YA. Pathogenicity of Some Fusarium Species Associated with Superficial Blemishes of Potato Tubers. Pol J Microbiol. 2013; 62(1):59–66.
Baylis C. Manual of microbiological methods for the food and drinks industry. Campden & Chorleywood Food Research Association Group; 2003.
Fiers M, Chatot C, Edel-Hermann V, Le Hingrat Y, YanougoKonate A, Gautheron N, Guillery E, Alabouvette C, Steinberg C. Diversity of microorganisms associated with atypical superficial blemishes of potato tubers and pathogenicity assessment. Eur J Plant Pathol. 2010; 128:353–371.
Moeller EM, Bahnweg G, Sandermann H, Geiger HH. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies and infected plant tissue. Nucleic Acid Res. 1992;20:6115-6116.
Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995;61(4):1323–1330.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725–2729.
Barnett EA, Fergus CL. The relation of extracellular amylase, mycelium, and time, in some thermophilic and mesophilic Humicola species. Mycopathol Mycol Appl. 1971;44(2):131-41.
Lui LH, Kushalappa AC. Response surface models to predict potato tuber infection by F. sambucinum from duration of wetness and temperature, and dry rot lesion expansion from storage time and temperature. Int J Food Microbiol. 2002; 76:19–25.
Lui LH, Vikram A, Abu-Nada Y, Kushalappa AC, Raghavan GSV, Al Mughrabi K. Volatile metabolic profiling for discrimination of potato tubers inoculated with dry and soft rot pathogen. Am J Potato Res. 2005;82:1–8.
Choiseul J, Allen L, Carnegie SF. Fungi causing dry tuber rots of seed potatoes in storage in Scotland. Potato Res. 2007;49: 241–253.
Peters RD, MacLeod C, Seifert KA, Martin RA, Hale LR, Grau CR, MacInnis S. Pathogenicity t potato tubers of Fusarium spp. isolated from potato, cereal and forage crops. Am J Potato Res. 2008b; 85:367–374.
Manici LM, Cerato C. Pathogenecity of Fusarium oxysporum f. sp. tuberosi isolates from tubers and potato plants. Potato Res. 1994;37:129–134.
Saber MM, Ashour AMA, Abdel Rahman TG, Alsaidi KI. Biochemical Changes of Potato Cultivars Due to Infection by Dry Rot Disease. Egypt. J. Phytopathol. 2013; 41(1):53-65.
Ashour AMA, Saber MM, Abdel Rahman TG, Alsaidi KI. Role of some bioagents and inducer resistance chemicals in management of potato dry-rot caused by Fusarium species.Int. J. Sci. Eng. Technol. 2017;8:1372-1380.
O’Donnell K, Cigelnik E.Two divergent intragenomicrDNA ITS2 types within a monophyletic lineage of the fungus Fusariumare nonorthologous. Mol Phylogenet Evol. 1997;7(1):103–116.
Ayoubi N, Soleimani MJ. Morphological and molecular identification of pathogenic Fusarium spp. on strawberry in Iran. Sydowia. 2016;68:163–171.
Chehri K. Molecular identification of pathogenic Fusarium species, the causal agents of tomato wilt in western Iran. J Plant Prot Res. 2016;56(2):143-148.
Waalwijk C, de Koning JR, Baayen RP, Gams W. Discordant groupings of Fusarium spp. from sections Elegans, Liseola and Dlaminia based on ribosomal ITS1 and ITS2 sequences. Mycologia. 1996;88(3):361-368.
Steinberg C, Edel V, Gautheron N, Abadie C, Vallaeys T, Alabouvette C. Phenotypic characterization of natural populations of Fusarium oxysporum in relation to genotypic characterization. Fems Microbiol Ecol. 1997;24:73–85.
Alabouvette C, Lemanceau P, Steinberg C. Recent advances in biological control of Fusarium wilts. J Pestic Sci. 1993;37:365-373.
Bakri Y, Jawhar M, Arabi MIE. Enhanced Amylase Production by Fusarium solani in Solid State Fermentation. Pak J Sci Ind Res Ser B Biol Sci. 2014;57(3):123-128.
Saleem A, Mohsen KHE. Production of amylase by fungi isolated from legume seeds collected in Almadinah Al munawwarah, Saudi Arabia. JTUSCI. 2014;8:90–97.
Aydoğdu H, Balkan B, Balkan S, Ertan F. Amylolytic Activities of Fungi Species on the screening medium adjusted to different pH. J Sci Technol. 2012;5:1-12.
Olivieri1 FP, Maldonado S, Tonon CV, Casalongue CA. Hydrolytic Activities of Fusarium solani and Fusarium solani f. sp. Eumartii Associated with the Infection Process of Potato Tubers. J Phytopathology. 2004;152,337–344.
Gawade L, Sarma VVSS, Rao YV, Hemalatha KPJ. Variation of bacterial metabolic arates and organic matter in the monsoon-affected tropical estuary (Godavari, India). Geomicrobiol J. 2017;34: 628-640.
Delgado JA, Schwarz PB, Gillespie J, Rivera-Varas VV, Secor GA. Trichothecene Mycotoxins Associated with Potato Dry Rot Caused by Fusarium graminearum. Phytopathology. 2010;100: 290-296.
Peters JC, Lees AK, Cullen DW, Sullivan L, Stroud GP and Cunnington AC. Characterization of Fusarium spp. Responsible for causing dry rot of potato in Great Britain. Plant Pathol. 2008a;57:262–271.
Estrada R, Gudmestad NC, Rivera VV, Secor GA. Fusarium graminearumas a dry rot pathogen of potato in the USA: prevalence, comparison of host isolate aggressiveness and factors affecting a etiology. Plant. Pathol. 2010;59:1114-1120.
Jawed S, Mehmood A, Hussain A, Jatoi ZA, Kubar AA, Nizamani MM Kaleri A A. Efficacy of Different Fungicides and Bio Control Agents Against Fusarium oxysporum, Causal Agent of Potato Dry Rot. Indian J Sci Technol (JST). 2019; 12(7):1-12.
SAS Institute Inc. SAS/STAT User’s Guide, Version 6, 12th Ed. SAS Institute, Inc. Cary, North Carolina, USA. 1996;2:846.
Bayona LG, Grajales A, Cárdenas ME, Sierra R, Lozano G, Garavito MF, Cepero de García MC, Bernal A, Jiménez P, Restrepo S. Isolation and characterization of two strains of Fusarium oxysporum causing potato dry rot in Solanum tuberosum in Colombia. Revista Iberoamericana de Micología. 2011;28(4): 166–72.
Chehri K, Mohamed NF, Salleh B, Latiffah Z. Occurrence and pathogenicity of Fusarium spp. on the potato tubers in Malaysia. African J. Agric. Res. 2011; 6(16):3706-3712.
Hoitink HA, Locke JC. An integrated approach to biological control of Fusarium species in containerized crops. Fusarium wilts of greenhouse vegetable and ornamental crops. APS Press, St Paul. 2012;109-15.
Esfahani MN. Present status of Fusarium dry rot of potato tubers in Isfahan (Iran). Indian Phytopath. 2006;59(2):142-147.
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