Determination of LD50, Fecundity and Locomotor Effects of Methanol Root Extract of Ximenia americana Linn, in Drosophila melanogaster

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Great David Bagu
Simeon Omale
Walter Mdekera Iorjiim
Mary O. Uguru
Steven Samuel Gyang


Aim: The study aimed at assessing the LD50, fecundity and locomotor effects of Ximenia americana L in Drosophila melanogaster.

Study Design: The study was an experimental design.

Place and Duration of Study: The study was done in the drosophila laboratory, Africa Centre of Excellence in Phytomedicine Research and Development (ACEPRD), University of Jos, Nigeria between November 2019 and Match, 2020.

Methods: The experimental animals (1-3 days old) of both sexes were exposed to different concentrations (1 mg 10 mg 50 mg, 100 mg, 200 mg, 250 mg 300 mg, 350 mg, 400 mg, 450 mg) of the plant extract per oral for seven days to determine the lethal dose, LD50. Thereafter, five days treatment was done using 50 mg, 100 mg 200 mg and 300 mg concentrations of the extract to assay for fecundity and locomotor effect in the fruit fly.

Results: The LD50 of the methanol extract of Ximenia americana in D. melanogaster was found to be 327.7 mg, this showed that the plant extract is relatively safe. Also, the result showed that both fecundity and locomotor behaviour of the treated and untreated flies was not significantly (P > 0.05) different. Thus, the extract at the used concentrations does not affects significantly both the reproductive capacity and the motor functions in the fruit fly.

Conclusion: All the tested concentrations used in this research are relatively safe (because of high LD50 327.7 mg) in the fruit fly and slightly increase the emergence of new fly with no noticeable negative effect in locomotor activity.

Fecundity, Drosophila melanogaster, Ximenia americana, negative geotaxis

Article Details

How to Cite
Bagu, G. D., Omale, S., Iorjiim, W. M., Uguru, M. O., & Gyang, S. S. (2020). Determination of LD50, Fecundity and Locomotor Effects of Methanol Root Extract of Ximenia americana Linn, in Drosophila melanogaster. Asian Journal of Biochemistry, Genetics and Molecular Biology, 5(2), 1-9.
Original Research Article


Fry JD, Heinsohn SL, Mackay TFC. Heterosis for viability, fecundity and male fertility in Drosophila melanogaster: Comparison of mutational and standing variation. Genet. Soceity Am. 1998;148(1): 1171–1188.

Barnes AI, Wigby S, Boone JM, Partridge L, Chapman T. Feeding, fecundity and lifespan in female Drosophila melanogaster. Proc. R. Soc. B. 2008;275:1675–1683.

Sonoshita M, Cagan RL. Modeling human cancers in Drosophila, 1st Ed. Elsevier Inc. 2017;121.

Nichols CD, Becnel J, Pandey UB. Methods to assay Drosophila behavior. J. Vis. Exp. 2012;61:1–5.

Nouhaud P, Mallard F, Poupardin R, Barghi N, Schlötterer C. High-throughput fecundity measurements in Drosophila. Sci. Rep. 2018;8(1):1–6.

Hanson FB, Ferris FR. A quantitative study of fecundity in Drosophila malenogaster. J. Exp. Zool. 1929;54(3):485–506.

Abolaji AO, et al. Ovotoxicants 4-vinylcyclohexene 1,2-monoepoxide and 4- vinylcyclohexene diepoxide disrupt redox status and modify different electrophile sensitive target enzymes and genes in Drosophila melanogaster. Redox Biol. 2015;5:328–339.

Baldal EA, Van Der Linde K, Van Alphen JJM, Brakefield PM, Zwaan BJ. The effects of larval density on adult life-history traits in three species of Drosophila. Mech. Ageing Dev. 2005;126:407–416.

José F, Monte Q, Leda T, De Lemos G. Ximenia americana: Chemistry, pharmacology and biological properties, a review; 2008.

Ribó G, Ocana J, Prevosti A. Effect of larval crowding on adult mating behaviour in Drosophila melanogaster. Heredity (Edinb). 1989;63:195–202.

Qiu S, Xiao C. Behavioral decoding of Drosophila locomotion in a circular arena. bioRxiv. 2017;1–29.

Kays R, Crofoot MC, Jetz W, Wikelski M. Terrestrial animal tracking as an eye on life and planet. Science. 2015;348(6240): aaa2478.

Eidhof I, Fenckova M, Elurbe DM, van de Warrenburg B, Nobau AC, Schenck A. High-throughput analysis of locomotor behavior in the Drosophila island assay. J. Vis. Exp. 2017;129:1–11.

Katsov AY, Freifeld L, Horowitz M, Kuehn S, Clandinin TR. Dynamic structure of locomotor behavior in walking fruit flies. Elife. 2017;6:1–32.

Murphey RM. Spatial discrimination performance of Drosophila melanogaster: Some controlled and uncontrolled correlates. Anim. Behav. 1969;17(PART 1):43–46.

Pyle DW. Correlated responses to selection for a behavioral trait in Drosophila melanogaster. Behav. Genet. 1978;8(4):333–340.

Ali YO, Escala W, Ruan K, Zhai RG. Assaying locomotor, learning and memory deficits in Drosophila models of neurodegeneration. J. Vis. Exp. 2011;49:1–5.

Madabattula ST, et al. Quantitative analysis of climbing defects in a Drosophila model of neurodegenerative disorders. J. Vis. Exp. 2015;100:1–9.

Lorenzo DN, Li MG, Mische SE, Armbrust KR, Ronum LPW, Hays TS. Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila. J. Cell Biol. 2010;189(1):143–158.

Agyigra AI, Ejiofor JI, Magaji MG, Yakubu Y. Evaluation of methanol stem-bark extract of Ximenia americana Linn (Olacaceae) for phytoconstituents and gastroprotection in rats. African J. Pharmacol. Ther. 2017;6(4):161–165.

Feyssa DH, Njoka JT, Asfaw Z, Nyagito MM. Uses and management of Ximenia americana, Olacaceae in semi-arid East Shewa, Ethiopia. Pak. J. Bot. 2012;44(4):1177–1184.

Orwa. Ximenia americana L. Agrofor. Database 4.0. 2009;29(03):1–5.

Sacande M, Vauteir H. Ximenia americana L. Seed Leafl. 2006;112:1–3.

Abubakar AA, Salka MN. Effects of methanol extract of Ximenia americana on sexual behaviour, testicular weight, sperm count and sperm morphology of wister rats. Ann. Biol. Res. 2011;2(1):107–113.

Shettar AK, Kotresha K, Kaliwal BB, Vedamurthy AB. Evaluation of in vitro antioxidant and anti-inflammatory activities of Ximenia americana extracts. Asian Pacific J. Trop. Dis. 2015;5(11):918–923.

Ogunleye DS, Ibitoye SF. Studies of antimicrobial activity and chemical constituents of Ximenia americana. Trop. J. Pharm. Reseasrch. 2003;2(2):239– 241.

Maikai VA. In vitro and in vivo evaluation of anti-trypanosomal activity of stem bark of Ximenia americana. Int. J. Biol. 2010;2(2):50–54.

Uchôa VT, Melo SCM, Carvalho AA, Goulard AESA, Chaves MH. Free radical scavenging ability of Ximenia americana L. stem bark and leaf extracts. J. Appl. Pharm. Sci. 2016;6(02):091–096.

Darcio J, et al. Physical-chemical characteristics and antioxidant potential of seed and pulp of Ximenia americana L. from the semiarid region of Brazil. African J. Biotechnol. 2015;14(20):1743–1752.

Voss C, Eyol E, Berger MR. Identification of potent anticancer activity in Ximenia americana aqueous extracts used by African traditional medicine. Toxicol. Appl. Pharmacol. 2006;211(3):177–187.

Le NHT, Malterud KE, Diallo D, Paulsen BS, Nergård CS, Wangensteen H. Bioactive polyphenols in Ximenia americana and the traditional use among Malian healers. J. Ethnopharmacol. 2011;139(2012):858–862.

Olanrewaju TO, Odumosu PO, Eyong KO. Anti-trypanosomal evaluation of Ximenia americana root bark and chromatographic-mass spectrometric profile GSC biological and pharmaceutical sciences anti-trypanosomal evaluation of Ximenia americana root bark and chromatographic -mass spectrometric profi. GSC Biol. Pharm. Sci. 2019;07(02):108–117.

Abbink J. Plant use among the Suri people of Southern Ethiopia: A system of knowledge in danger? AAP. 2002;70:199–206.

Abbink J. Indigenous knowledge and development monitor. PRELUDE HA 37; 1995.

Iorjiim WM, Omale S, Etuh MA, Bagu GD, Ogwu SO, Gyang SS. EFV b-HAART increases mortality, locomotor deficits and reduces reproductive capacity in Drosophila melanogaster. J. Adv. Biol. Biotechnol. 2020;23(1):26–38.

Charpentier G, et al. Lethal and sublethal effects of imidacloprid, after chronic exposure, on the insect model Drosophila melanogaster. Environ. Sci. Technol. 2014;48:4096–4102.

Mohammad F, Singh P, Sharma A. A Drosophila systems model of pentylenetetrazole induced locomotor plasticity responsive to antiepileptic drugs. BCM Syst. Biol. 2009;3(11):1–17.

Jeruto P, Lukhoba C, Ouma G, Otieno D, Mutai C. An ethnobotanical study of medicinal plants used by the Nandi people in Kenya. J. Ethnopharmacol. 2008;116(2):370–376.

Ezuruike UF, Prieto JM. The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations. J. Ethnopharmacol. 2014;155(2):857–924.

Maikai VA, Kobo PI, Adaudi AO. Acute toxicity studies of aqueous stem bark extract of Ximenia americana. African J. Biotechnol. 2008;7(10):1600–1603.

Bagu GD, et al. In vivo antioxidant and toxicity properties of methanol root extract of Ximenia americana L. (Olacaceae) in Drosophila melanogaster. Int. J. Eng. Appl. Sci. Technol. 2020;4(12):59–66.