Molecular Diversity Analysis of Indigenous Potato Cultivars Using RAPD Markers
Asian Journal of Biochemistry, Genetics and Molecular Biology,
The study was undertaken to identify genotypic diversity at molecular level of potato for varietal improvement program at the Advanced Plant Breeding Laboratory, Department of Genetics and Plant Breeding, Bongobondhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh. Eight cultivars of potato viz. Lalpakri, Sindurkouta, Indurkani, Ausha, Patnai, Sadaguti, Shilbilati, Challisha were collected from Bangladesh Agricultural Research Institute (BARI), for studying genotyping divergence. Genomic DNA was extracted from young leaves of the cultivars and PCR reaction was performed. The PCR amplified DNA profile was visualized on agarose gel, staining with ethidium bromide. Three RAPD primers named OPA 01, OPA 03 and OPD 18 were used to evaluate the genetic diversity of potato varieties. All the primers were polymorphic and the primers produced the highest number of alleles. The genetic diversity value in Lalpakri with all cultivars except Sadaguti was found to have the highest genetic distance (1.0). The amount of genetic diversity within potato germplasm is quite distinct as revealed by the genetic similarity coefficients. The results indicate that, high level of genetic distance exists among the cultivars. The Primers OPA 01, OPA 03 and OPD 18 showed the highest level of genetic diversity and PIC value while the Lalpakri and Sadaguti cultivars had the highest genetic distance among other cultivars which could be used for further potato breeding program.
- potato and RAPD marker.
How to Cite
Polzerova H. The use of molecular genetic tecniques to potato variety identification. Rostlinna Výroba. 2001; 47(11):78-87.
Williams JGK, Kubelik KJ, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphism amplified by arbitrary primers are useful genetic markers. Nucleic Acids Research. 1990;18:6531-6535.
Sharma K, Mishra AK, Misra RS. A simple and efficient method for extraction of genomic DNA from tropical tuber crops. African Journal of Biotechnology. 2008; 7(8):1018-1022.
Miller JT, Spooner D. Collapse of species boundaries in the wild potato Solanum brevicaule complex (Solanum sp. Sect. Petota): molecular data. Plant Systematic and Evolution. 1999;214:103-130.
Isenegger DA, Taylor PJ, Ford R, Franz P, Mcgregor GR. DNA fingerprinting and genetic relationships of potato cultivars (Solanum tuberosum L.) commercially grown in Australia. Australian Journal of Agricultural Research. 2001;52:911- 918.
Moisan TM, Hingrat YL, Kerlan MC. Potato cultivars identification using molecular markers. Acta Horticulturae. 2001;546:471-477.
Sun GG, Wang-pruski M, Mayich M, De Jong H. RAPD and pedigree-based genetic diversity estimates in cultivated diploid potato hybrids. Theoretical and Applied Genetics. 2003;107:110-115.
Spooner DM, McLean KG, Fau–Ramsay K R, Ramsay G. A single domestication for potato based on multi-locus amplified fragment length. Proceedings of the National Academy of Sciences of the United States of America. 2005;102:1494-1499.
Rocha AE, Luciano VP, Humberto HC and Claudia TG, Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. Crop Breeding and Applied Biotechnology. 2010;10:1984-7033.
Ghislain MD, Andrade F, Rodríguez RJ, Hijmans, Spooner, DM. Genetic analysis of the cultivated potato Solanum tuberosum L. Phureja group using RAPDs and nuclear SSRs. Theoretical and Applied Genetics. 2006;113:1515-1527.
Das AB, Mohanty IC, Mahapatra D, Mohanty S, Ray A. Genetic variation of Indian potato (Solanum tuberosum L.) genotypes using chromosomal and RAPD markers. Crop Breeding and Applied Biotechnology. 2010;10:238-246.
Murray MG, Thampson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 1980;8: 4321-4325.
Nei M. Genetic distance between populations. The American Naturalist. 1972;106: 283-292.
Sneath PHA, Sokal RR. Numerical taxonomy: the principles and practice of numerical classification. San Francisco: Freeman. 1973;573.
Bianchi VJ, Fachinello JC, Schuch MW. RAPDs na caraterizacao geneticomolecular eno estudo da variabilidade genetica de cultivares de ameixeira. Revista Brasileira de Fruticultura. 2003;25:272-274.
Abstract View: 219 times
PDF Download: 196 times