Assessment of the Inherent in-vitro Antioxidant Potential of Commelina benghalensis Leaf Extract

Main Article Content

Tebekeme Okoko

Abstract

Commelina benghalensis is a troublesome but exotic weed native to the African and Asian subregions used traditionally for the treatment and management of various disorders. The aim of this study was to investigate the potential antioxidant activity of the methanolic leaf extract of Commelina benghalensis using various in vitro models. This was done by investigating the ability of the extract to scavenge hydrogen peroxide and hydroxyl radical. Other activities assessed were the reducing ability, ability to inhibit erythrocyte damage and reduce ferrous-ascorbate induced lipid peroxidation on bovine liver and egg yolk homogenates. The results revealed that the plant extract possessed significant hydrogen peroxide and hydroxyl radical scavenging abilities. The extract also possessed  significant ability to reduce ferric ions and molybdate VI. The methanolic extract also significantly inhibited hydrogen peroxide-induced erythrocyte hemolysis and lipid peroxidation. Lipid peroxidation in bovine liver and egg yolk homogenates induced       by the ferrous-ascorbate system was also reduced by the extract. In many instances, the effect of the extract was concentration-dependent. (p < 0.05). This antioxidant activity of the extract is ascribed to the phytochemicals which probably acted in synergy thus the Commelina benghalensis leaves could be exploited both nutraceutically and pharmacologically.

Keywords:
Commelina benghalensis, hydrogen peroxide, lipid peroxidation, erythrocyte, antioxidant

Article Details

How to Cite
Okoko, T. (2021). Assessment of the Inherent in-vitro Antioxidant Potential of Commelina benghalensis Leaf Extract. Asian Journal of Biochemistry, Genetics and Molecular Biology, 6(4), 25-32. https://doi.org/10.9734/ajbgmb/2020/v6i430159
Section
Original Research Article

References

Ibrahim J, Ajaegbu VC, Egharevba HO. Pharmacognostic and phytochemical analysis of Commelina benghalensis L. Ethnobotanical Leaflets. 2010;14:610-615.

Culpepper AS, Flanders JT, York AC, Webster TM. Tropical spiderwort (Commelina benghalensis) control in Glyphosate-resistant cotton1. Weed Tech. 2004;18(2):432-436.

Kabir MS, Hasanat A, Chowdhury TA, Rashid MM, Hossain MM, Ahmed S. Study of antidiarrheal and anthelmintic activity methanol extract of Commelina benghalensis leaves. Afr J Pharm Pharmacol. 2016;10(32):657-664.

Mbazima VG, Mokgotho MP, February F, Rees DJ, Mampuru LJ. Alteration of Bax-to-Bcl-2 ratio modulates the anticancer activity of methanolic extract of Commelina benghalensis (Commelinaceae) in Jurkat T cells. Afri J Biotechnol. 2008;7(20):3569-3576.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989; 10(6):1003-1008.

Yu W, Zhao Y, Shu B. The radical scavenging activities of radix puerariae isoflavonoids: A chemiluminescence study. Food Chem. 2004;86(4):525-529.

Oyaizu M. Studies on products of browning reaction. The Jap J Nutr Diet. 1986; 44(6):307-315.

Okoko T, Ere D. Reduction of hydrogen peroxide–induced erythrocyte damage by carica papaya leaf extract. Asian Pacific J Trop Biomed. 2012;2(6):449-453.

Jayaprakasha GK, Jena BS, Negi PS, Sakariah KK. Evaluation of antioxidant activities and antimutagenicity of turmeric oil: A byproduct from curcumin production. Zeitschrift Für Naturforschung C. 2002; 57(9-10):828-835.

Yoshiyuki K, Michinori K, Tadato T, Shigeru A, Hiromichi O. Studies on scutellariae radix. IV. Effects on lipid peroxidation in rat liver. Chem Pharm Bull. 1981;29:2610-2617.

Ndoni S, Ere D, Okoko T. Assessment of the in vitro anti-lipid peroxidative activity of costus afer stem extract. Oxidants Antiox Med Sci. 2017;6(2):30-34.

Graves DB. The emerging role of reactive oxygen and nitrogen species in redox biology and some implications for plasma applications to medicine and biology. J Physics D: Appl Physics. 2012;45(26): 263001.

Roberts RA, Smith RA, Safe S, Szabo C, Tjalkens RB, Robertson FM. Toxicological and pathophysiological roles of reactive oxygen and nitrogen species. Toxicol. 2010;276(2):85-94.

Figueira TR, Barros MH, Camargo AA, Castilho RF, Ferreira JC, Kowaltowski AJ, Sluse FE, Souza-Pinto NC, Vercesi AE. Mitochondria as a source of reactive oxygen and nitrogen species: From molecular mechanisms to human health. Antioxid Redox Signal. 2013;18(16):2029-74.

Buettner GR, Ng CF, Wang M, Rodgers VG, Schafer FQ. A new paradigm: Manganese superoxide dismutase influences the production of H2O2 in cells and thereby their biological state. Free Rad Biol Med. 2006;41(8):1338-1350.

Lennicke C, Rahn J, Lichtenfels R, Wessjohann LA, Seliger B. Hydrogen peroxide–production, fate and role in redox signaling of tumor cells. Cell Comm Signal. 2015;13(1):1-9.

Jian Z, Li K, Liu L, Zhang Y, Zhou Z, Li C, Gao T. Heme oxygenase-1 protects human melanocytes from H2O2-induced oxidative stress via the Nrf2-ARE pathway. J Invest Dermatol. 2011;131(7):1420- 1427.

Kaneko S, Kawakami S, Hara Y, Wakamori M, Itoh E, Minami T, Takada Y, Kume T, Katsuki H, Mori Y, Akaike A. A critical role of TRPM2 in neuronal cell death by hydrogen peroxide. J Pharmacol Sci. 2006;101(1):66-76.

Aruoma OI. Free radicals and international nutrition. Asia Pacific J Clin Nutr. 1999; 8:53-63.

Zhu QY, Holt RR, Lazarus SA, Orozco TJ. Inhibitory effects of cocoa flavanols and procyanidin oligomers on free radical-induced erythrocyte hemolysis. Exp Biol Med. 2002;227(5):321-329.

Kolanjiappan K, Manoharan S, Kayalvizhi M. Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clinica Chimica Acta. 2002;326(1-2):143-149.

Tavazzi B, Di Pierro D, Amorini AM, Fazzina G, Tuttobene M, Giardina B, Lazzarino G. Energy metabolism and lipid peroxidation of human erythrocytes as a function of increased oxidative stress. Eur J Biochem. 2000;267(3):684-689.

Herken H, Uz E, Özyurt H, Söğüt S, Virit O, Akyol Ö. Evidence that the activities of erythrocyte free radical scavenging enzymes and the products of lipid peroxidation are increased in different forms of schizophrenia. Mol Psychiatry. 2001;6(1):66-73.

Schafer FQ, Qian SY, Buettner GR. Iron and free radical oxidations in cell membranes. Cell Mol Biol (Noisy-le-Grand, France). 2000;46(3):657-662.

Embuscado ME. Spices and herbs: Natural sources of antioxidants–a mini review. J Func Foods. 2015;18:811-819.

Neergheen VS, Bahorun T, Taylor EW, Jen LS, Aruoma OI. Targeting specific cell signaling transduction pathways by dietary and medicinal phytochemicals in cancer chemoprevention. Toxicol. 2010; 278(2):229-241.

Bosch R, Philips N, Suárez-Pérez JA, Juarranz A, Devmurari A, Chalensouk-Khaosaat J, González S. Mechanisms of photoaging and cutaneous photocarcinogenesis and photoprotective strategies with phytochemicals. Antioxidants. 2015;4(2): 248-268.

Fiedor J, Burda K. Potential role of carotenoids as antioxidants in human health and disease. Nutrients. 2014;6(2): 466-488.

Cör D, Knez Ž, Knez Hrnčič M. Antitumour, antimicrobial, antioxidant and antiacetylcholinesterase effect of ganoderma lucidum terpenoids and polysaccharides: A review. Molecules. 2018;23(3):649.

Rehman S, Khan H. Advances in antioxidant potential of natural alkaloids. Curr Bioac Comp. 2017;13(2): 101-108.

Anusuya NA, Gomathi RA, Manian SE, Sivaram VE, Menon AN. Evaluation of basella rubra L. Rumex nepalensis spreng and Commelina benghalensis L. For antioxidant activity. Int J Phar Pharmaceut Sci. 2012;4:714-720.