The Effect of Thaumatococcus danielli Leaf Extracts on Immunological and Oxidative Stress Markers in Rat
Asian Journal of Biochemistry, Genetics and Molecular Biology,
This work demonstrated the immunological and antioxidant activities of Thaumatococcus daniellii leaf extracts and their potential as the pharmaceutical factor for the treatment of some pathological disorders such as cancer, leucopenia, cells inflammation, dietary, atherosclerosis, neurodegenerative disease and other immunological and oxidative stress disorders.36 albino rats of average weight 140 ± 5 g were randomly grouped into 6 with six rats per group. The rats in group A (positive control) received only 0.1% DMSO. The rats in the negative control (group B) received pyrogallol (immunosuppressor) + 0.1% DMSO for 7 days and challenged with 0.1ml Sheep Red Blood Cell (SRBC). The test groups (C to F) also received pyrogallol + 0.1% DMSO for 7 days and were also challenged with SRBC for a period of 72hours after which they were treated with High and Low doses of either petroleum ether or water extracts of Thaumatococcus danielii for 21days. The assessment of immunomodulatory activity was carried out using Carbon Clearance Test. Haematological/ immunological parameters, Spleen and Plasma antioxidant parameters like Catalase (CAT), Superoxide dismutase (SOD), Glutathione Peroxidase (GPX) Activities, Lipid Peroxidation (LPO) and Malonaldehyde (MDA) concentrations were also determined. Result unveiled a significant (p<0.05) increase in the phagocytic index of both high doses of water and petroleum ether extracts compared to the negative control group. There were no significant (p<0.05) changes in the Heamatological parameters and plasma catalase, but there was a significant change in the white blood cell (WBC) count and also an improvement in the spleen and plasma antioxidant parameters. Further study is required to understand the mechanism of action of both extracts in order to exploit it in immunomodulation.
- oxidative stress
- thaumatococcus danielli
How to Cite
Arowosoge O. Economic analysis of Thaumatococcus danielli (Benn.) Benth. (miraculous berry) in Ekiti State, Nigeria; 2006.
Adebayo GJ, Kolawole LA. In vitro activity of Thaumatococcus daniellii and Megaphrynium macrostachyum against spoilage fungi of white bread and Eba, an indigenous staple food in southern Nigeria. African Journal of Microbiology Research. 2010;4:1076-1081.
Ojekale, Anthony B, et al. Volatile constituents, antioxidant and insecticidal activities of essential oil from the leaves of Thaumatococcus danielli (Benn.) Benth. From Nigeria. Iosr Journal of Pharmacy. 2013;3.3:01-05.
Amusa T, Jimoh S, Azeez I. Prevalence, utilization and conservation strategies for non-timber forest products in South Western zone of Nigeria. Resources and Environment. 2012;2:46-54.
Srikumar R, Parthasarathy NJ, Manikandan S, Narayanan GS, Sheeladevi R. Effect of Triphala on oxidative stress and on cell-mediated immune response against noise stress in rats. Molecular and cellular biochemistry. 2006;283:67-74.
Hirokawa K, Utsuyama M. Animal models and possible human application of immunological restoration in the elderly. Mechanisms of ageing and development. 2002;123:1055-1063.
Gate L, Paul J, Ba GN, Tew K, Tapiero H. Oxidative stress induced in pathologies: The role of antioxidants. Biomedicine & Pharmacotherapy. 1999;53:169-180.
Knight JA. Free radicals, antioxidants, and the immune system. Annals of Clinical & Laboratory Science. 2000;30:145-158.
Devasagayam T, Sainis K. Immune system and antioxidants, especially those derived from Indian medicinal plants; 2002.
Gupta Y, Sharma M, Chaudhary G. Pyrogallol-induced hepatotoxicity in rats: a model to evaluate antioxidant hepatoprotective agents. Methods and findings in experimental and clinical pharmacology. 2002;24:497-500.
Archer D. Immunotoxicology of food borne substances: an overview. Journal of Food Protection. 1978;41:983-988.
Joharapurkar AA, et al. Pyrogallol: A novel tool for screening immunomodulators. Indian Journal of Pharmacology. 2004; 36.6:355.
Lorke D. A new approach to practical acute toxicity testing. Archives of Toxicology. 1983;54:275-287.
Burrel T. Parks plan and people, Council of Europe, Strasbourg; 1987.
Yakubu M, Akanji M, Oladiji A. Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem in male albino rats. Asian Journal of Andrology. 2005;7:399-404.
Jayathirtha M, Mishra S. Preliminary immunomodulatory activities of methanol extracts of
Eclipta alba and Centella asiatica. Phytomedicine. 2004;11:361-365.
Gokhale A, Damre A, Saraf M. Investigations into the immunomodulatory activity of Argyreia speciosa. Journal of Ethnopharmacology. 2003;84:109-114.
Mullen P. Zinc sulphate turbidity test as an aid to diagnosis. Veter Ann. 1975;15:451-455.
Keil A, Müller MM, Gruber T, Wienbruch C, Stolarova M, Elbert T. Effects of emotional arousal in the cerebral hemispheres: A study of oscillatory brain activity and event-related potentials. Clinical neurophysiology. 2001;112:2057-2068.
Gregory EM, Fridovich I. Oxygen metabolism in Lactobacillus plantarum. Journal of bacteriology. 1974;117:166-169.
Aebi H. Catalase in vitro, in Methods in enzymology Elsevier. 1984;121-126.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine. 1967;70:158-169.
Rotruck JT, Pope AL, Ganther HE, Swanson A, Hafeman DG, Hoekstra W Selenium: Biochemical role as a component of glutathione peroxidase. Science. 1973;179:588-590.
Fridovich I. Superoxide dismutases. Advances in enzymology and related areas of molecular biology. 1986;58:61-97.
Stocks J, Dormandy T. The autoxidation of human red cell lipids induced by hydrogen peroxide. British journal of haematology. 1971;20:95-111.
Patel P, Asdaq SMB. Immunomodulatory activity of methanolic fruit extract of Aegle marmelos in experimental animals. Saudi Pharmaceutical Journal. 2010;18:161- 165.
Houghton AN, Guevara-Patiño JA. Immune recognition of self in immunity against cancer. The Journal of clinical investigation. 2004;114:468-471.
Bonacorsi C, Da Fonseca LM, Raddi MSG, Kitagawa RR, Vilegas W. Comparison of Brazilian plants used to treat gastritis on the oxidative burst of Helicobacter pyloristimulated neutrophil. Evidence-Based Complementary and Alternative Medicine; 2013.
Swirski FK, Nahrendorf M, Etzrodt M, Wildgruber M, Cortez-Retamozo V, Panizzi P, et al. Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science. 2009; 325:612-616.
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 1974;47:469-474.
Kolanjiappan K, Manoharan S, Kayalvizhi M. Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clinica Chimica Acta. 2002;326:143-149.
Al-Mustafa AH. In vitro Study Involving the Comparative Effect of Heavy Metal Ions on Antioxidant Enzymes Activity and Lipid Peroxide Levels in Human Erytrocytes. Pakistan Journal of Biological Sciences. 2006;9:2586-2592.
Abstract View: 65 times
PDF Download: 50 times