Effect of Decryodes edulis (African Black Pear) Aqueous Leaf Extract on Liver Enzyme Markers of Acetaminophen-induced Hepatotoxicity in Wistar Rats
Asian Journal of Biochemistry, Genetics and Molecular Biology,
Page 48-56
DOI:
10.9734/ajbgmb/2022/v11i230265
Abstract
Aim: This study investigated the effect of aqueous extract of Dacryodes edulis (African Black Pear) on liver enzyme markers associated with acetaminophen-induced hepatotoxicity.
Method: Qualitative phytochemical screening was done on the aqueous leaves extract of D. edulis and in vivo experiment was carried out afterwards. 20 adult male Wistar rats were used which were randomized into 5 groups of 4 rats each. Group 1 served as the positive control and were fed with rat pellets and water only, Group 2 served as acetaminophen control and were fed with rat pellets and water and then treated with 2 g/kg acetaminophen only, Group 3, 4 and 5 was served 2g/kg acetaminophen and treated with 100mg/kg, 150 mg/kg and 200 mg/kg of stock solution of the plant extract in addition to rat pelleted feed and water ad libitum. The experimental process lasted for 14 days after which blood samples were collected for liver enzyme assay. Alanine aminotransferase (ALT), asparatate aminotransferase (AST) and alkaline phosphatase (ALP) were assayed using Randox Kits.
Results: Result obtained revealed that aqueous extract of Dacryodes edulis significantly (p<0.05) reduced acetaminophen hepatotoxicity at a dose dependent rate evident by the reduction in the levels of AST, ALT, ALP, Urea and creatinine.
Conclusion: The result showed the hepatoprotective effect of Dacryodesedulis against toxicity induced by acetaminophen induction in the liver and this is due to the phytochemicals present in the leaf part of Dacryodes edulis. Hence, Dacryodes edulis has hepatoprotective potential against acetaminophen liver damage.
Keywords:
- Decryodes edulis
- aqueous leaves extract
- liver ENZYME MARKers
- acetaminophen
- hepatotoxicity
- phytochemicals
How to Cite
References
Ibrahim T, Agnihotri S, Agnihotri AK. Paracetamol Toxicity-An overview. EmergencyMedicine. 2013;3:158.
Yoon E, Babar A, Choudhary M, Kutner M, Pyrsopoulos N. Journal Clinical Transplant and Hepatology. 2016;4(2):131-142.
Hutchinson J, Dalziel JM. Flora of West Tropical Africa. Vol. 1, Crown Agents for Overseas Government, London. 1958; 763
Burkill HM. The Useful Plants of West Tropical Africa. Vol. 1, Royal Botanic Gardens, Kew. 1985;960
ISBN: 094764301X
Verheij EWM. Dacryodes edulis (G.Don) H.J.Lam. [Internet] Record from PROTA4U. Oyen, L.P.A. & Lemmens, R.H.M.J. (Editors). PROTA (Plant Resources of Tropical Africa / Ressources végétales de l’Afrique tropicale), Wageningen, Netherlands; 2002.
Available:http://www.prota4u.org/search.asp, Accessed 7th December, 2021.
Medenbach de Rooy JM. The biodiversity of African plants. Proceedings of the 14th AETFAT Congress, 22-27 August, Wageningen, Netherlands, Aug. 22-27, Kluwer Academic Publishers, Dordrecht, Netherlands. 1994;684-695
OkaforJC. Varietal delimitation in Dacryodesedulis (G. Don) H.J. Lam (Burseraceae). Int. Tree Crops J. 1983;2:255-265
Waruhiu AN, Kengue J, Atangana AR, Tchoundjeu Z, Leakey RRB.Domestication of Dacryodesedulis: 2. Phenotypic variation of fruit traits from 200 trees from four populations in the humid lowlands of Cameroon. J. Food Agric. Environ. 2004;2: 340-346.
Okwu DE, Nnamdi FU.Evaluation of the chemical composition of Dacryodesedulis sp. check for this species in other resources and raphiahookerimann and wendl exudates used in herbal medicine in South Eastern Nigeria. Afr. J. Tradit. Compl. Altern. Med. 2008;5:194-200
Ajibesin KK. Dacryodesedulis (G. Don) H. J. Lam: A Review on Its Medicinal, Phytochemical and Economic Properties. Research Journal of Medicinal Plants. 2011;5:32-34.
Agbor GA, Kuate D, Oben JE. Medicinal plants can be good source of antioxidants: Case study in Cameroon. Pak. J. Biol. Sci. 2007;10:537-544.
Bhatt ID, Rawat S, Rawal RS. Antioxidants in Medicinal plants. Biotechnology for Medicinal plants – Micropropagation and Improvement. 2013;295–236
Cowman MM. Plant products as antimicrobial agents. Clinicasl Microbiology Review. 1999;12:564-582.
Adesokan AA, Yakubu MT, Owoyele BV, Akanji MA, Soladoye A, Lawal OK. Effect of administration of aqueus and ethnolic extracts of Enantiachlorantha stem bark on brewer’s yeast-indicedpyresis in rats. African Journal of Biochemical Research. 2008; 2(7):165-169.
Singh R. Medicinal Plants: A Review. Journal of Plant Sciences. Special Issue: Medicinal Plants. 2015;3(11):50-55
Harbone JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Springer Science and Business Media, Berlin, Heidelberg, 3rd Edition; 1998.
Uahomo PO, Isirima JC, Akoko S. Evaluation of Phytochemicals and Bioactive Properties in Leaf and Root Parts of Cyathula prostrata (Pasture Weed) – A Qualitative and Quantitative Analysis. Asian Plant Research Journal. 2022;9(3): 8-16. DOI:https://doi.org/10.9734/aprj/2022/v9i330207
Chimaobi J. Ononamadua, Adamu J. Alhassan, Aminu Ibrahim, Abdullahi A. Imam, Godwin O. Ihegboroa, Alowonle T. Owolarafea, Obiajulu C. Ezeigwe, Mohammed K. Atiku, Mohammed S. Sule. Toxicological study of aqueous-methanol solvent fraction of methanol extract of Dacryodes edulis leaves. Toxicology Reports. 2020;7:909-918
Olaleye MT, Adegboye OO, Akindahunsi AA. Alchornea cordifolia Extract Protects Wistar Albino Rats against Acetaminophen-Induced Liver Damage. African Journal of Biotechnology. 2006;5: 2439-2445
Ibekwe SE, Uwakwe AA, Monanu MO. In view effects of sodium benzoate on plasma AST and ALP of wistar albino rats. Scientific Research & Essay. 2007;2(1): 010-012.
Haussament TU. Quantitative determination of serum alkaline phospha-tase. Clin. Chem. Acta. 1977;35: 271-273.
Abirami A, Nagarani G, Siddhuraju P. Hepatoprotective effect of leaf extracts from Citrus hystrix and C. maxima against paracetamol induced liver injury in rats. Food Science and Human Wellness; 2015.
DOI:http://dx.doi.org/10.1016/j.fshw.2015.02.002
Chen C, Krausz KW, Shah YM, Idle JR, Gonzalez FJ, et al. Serum metabolomics reveals irreversible inhibition of fatty acid beta-oxidation through the suppression of PPAR alpha activation as a contributing mechanism of acetaminophen-induced hepatotoxicity.Chem Res Toxicol. 2009;22: 699–707.
Bhattacharyya S, Pence L, Beger RD, Chaudhuri S, McCullough SS, Kan Y, Simpson PM, Hennings L, Hinson JA, James LP, et al. Acylcarnitine profiles in acetaminophen toxicity in the mouse: comparison to toxicity, metabolism and regeneration. Metabolites. 2013;3:602-622.
Darbar S, Bhattacharya A, Darbar SCS, Bhattacharya A, ChattopadhyayS. et al. Antihepatoprotective potential of livina, a polyherbal preparation on paracetamol induced hepatotoxicity: a comparison with 395 silymarin. Asian J Pharm Clin Res. 2011;4(1):72–77.
Sasidharan S, Aravindran S, Latha LY, Vijenthi R, Saravanan D, Amutha S. et al. In vitro antioxidant activity and hepatoprotective effects of lentinulaedodes against paracetamol induced hepatotoxi-city. Molecules. 2010;15(6): 4478-448.
Kamgaing, MariusTre´sorWego, Marie Alfrede Mvondo, Sylviane Laure PoualeuKamani, Ste´phane Minko Essono, and Sylvie Lea Wansi Ngnokam. The Aqueous Extract of Dacryodesedulis (Burseraceae) Leaves Inhibits Cell Proliferation Induced by Estradiol on the Uterus and Vagina of Ovariectomized Female Wistar Rats. Hindawi, Advances in Pharmacological and Pharmaceutical Sciences; 2020.
DOI:https://doi.org/10.1155/2020/886928 1
Johns T. Dietary, diversity, global change and human health. Proceedings of the Symposium Managing Biodiversity in Agricultural Ecosystems, Nov. 8-10, Montreal, Canada. 2001;1-11
Eidangbe, George O, Ikechukwu O. Obasi, Antoinette C. Okaka, Rachel C. Eidangbe, Olarewaju M. Oluba Attenuation of Carbon Tetrachloride - Induced Hepatoxicity by Dacryodesedulis Seeds Ethanolic Extract in Male Wistar Rats. Biointerface Research in Applied Chemistry. 2021;11(2):9490- 9500 .
DOI:https://doi.org/10.33263/BRIAC112.94909500
Orhue NEJ, Adaikpoh MA, Odude O, Iseghohi SO. Prevention of Carbon Tetrachloride-induced Hepatic Steatosis and Cellular Damage by Aqueous Extract of DacryodesedulisSeeds in Wistar Rats. Bio-Research. 2015;13:885–888.
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