Therapeutic Role of Crinum glaucum Bulb against Haematological and Hepatic Enzyme Alterations in a Rat Model
Kehinde Oluwatomilola Olatunji
Drug Discovery Unit, Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, PMB-0001, LASU Post Office, Lagos – Badagry Expressway, Lagos, Nigeria.
Oluwaseyi Abiola Ogunrinola
Drug Discovery Unit, Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, PMB-0001, LASU Post Office, Lagos – Badagry Expressway, Lagos, Nigeria.
Olufunmilayo Olabisi Ogunrinola *
Drug Discovery Unit, Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, PMB-0001, LASU Post Office, Lagos – Badagry Expressway, Lagos, Nigeria.
Olamilekan Jimoh Igbalaye
Drug Discovery Unit, Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, PMB-0001, LASU Post Office, Lagos – Badagry Expressway, Lagos, Nigeria.
Adesewa Esther Adeniyi
Drug Discovery Unit, Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, PMB-0001, LASU Post Office, Lagos – Badagry Expressway, Lagos, Nigeria.
Aminat Abike Ahmad
Drug Discovery Unit, Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, PMB-0001, LASU Post Office, Lagos – Badagry Expressway, Lagos, Nigeria.
Benjamin Segun Aribisala
Department of Computer Science, Faculty of Science, Lagos State University, Ojo, PMB-0001, LASU Post Office, Lagos – Badagry Expressway, Lagos, Nigeria.
Babajide Olufemi Elemo
Drug Discovery Unit, Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, PMB-0001, LASU Post Office, Lagos – Badagry Expressway, Lagos, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: Several parts of medicinal plants have been known to have many health benefits. One of those plants is the Crinum glaucum (CG) bulb. Therefore, this research was undertaken to investigate the therapeutic role of an aqueous extract of CG bulb (aeCGb) against lipopolysaccharide (LPS)-induced haematological and hepatic enzyme alterations in male and female rat models.
Study Design and Methodology: Twenty-five male and twenty-five female rats were divided randomly into five groups (n = 5) each. Group 1 is the control group. Group 2 was administered with 1000 mg/kg body weight of aeCGb. Group 3 was exposed to 4 ml/kg body weight of LPS for 4 hours. Group 4 was administered LPS (4 hours) and 1000 mg/kg body weight of aeCGb. Group 5 was administered 1000 mg/kg body weight of aeCGb and LPS for 4 hours. The albumin, total protein, bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and gamma-glutamyl transferase (hepatic enzyme) concentrations and haematological parameters were analysed spectrophotometrically.
Results: The hallmark of LPS is its ability to decrease the concentration of hepatic enzymes and the levels of haematological parameters, as observed. The levels of albumin, total protein, and direct and total bilirubin were significantly (P≤ .05) increased in the aeCGb-treated groups. However, treatment with aeCGb reverses the damaging effect of LPS on hepatic enzyme concentration.
Conclusion: The results suggest that aeCGb has a therapeutic role in the LPS-induced alterations by correcting the concentration of hepatic enzyme function as well as regulating the levels of haematological parameters.
Keywords: Alkaline phosphatase, lipopolysaccharide, Crinum glaucum, alleviative; hepatic