Formulation of Silver Nanoparticles from Grewia Asiatica Leaf Extract and their Biomedical Applications

Syed Bilal Hussain *

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Shabnam Iqbal

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Sadaf Noor

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Muhammad Zubair

Department of Forestry and Range Management, FAS&T, Bahauddin Zakariya University, Multan, Pakistan.

*Author to whom correspondence should be addressed.


Nanotechnology is a well-advanced area and several types of nanoparticles have been synthesized. Different types of nanoparticles have been synthesized so far using different techniques. It is most practical to produce nanoparticles from plant extracts for use in medicine and other applications. In this study, silver nanoparticles were synthesized by Grewia asiatica leaf extract. Synthesized nanoparticles were characterized by different techniques including Ultraviolet-visible (UV) spectroscopy, X-ray diffraction (XRD), Dynamic light scattering (DLS), and Scanning electron microscopy (SEM). Results showed a characteristic peak of silver nanoparticles in UV-region at 425nm, while DLS analysis revealed the z-average size of synthesized particles i.e., 461.6nm and -28.4mV zeta potential. SEM analysis revealed the amorphous surface morphology. The antibacterial and antioxidant activity of silver nanoparticles were also evaluated. Silver nanoparticles showed significant antibacterial activity against both gram-positive and gram-negative bacteria. A maximum zone of inhibition of 16mm diameter was observed against Bacillus subtilis and Klebsiella pneumonia. While results of antioxidant activity showed a 43.27μg/ml IC50 value. In conclusion, Grewia asiatica leaf extract is a good source to synthesize silver nanoparticles. Also, green synthesized silver nanoparticles could act as potential antibacterial and antioxidant agents.

Keywords: Nanotechnology, biomedical application, Grewia sp, extract

How to Cite

Hussain , Syed Bilal, Shabnam Iqbal, Sadaf Noor, and Muhammad Zubair. 2023. “Formulation of Silver Nanoparticles from Grewia Asiatica Leaf Extract and Their Biomedical Applications”. Asian Journal of Biochemistry, Genetics and Molecular Biology 15 (3):45-55.


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Khandel P, et al. Biogenesis of metal nanoparticles and their pharmacological applications: present status and application prospects. Journal of Nanostructure in Chemistry. 2018;8:217-254.

Rajkumar R, et al. Cyber-physical systems: the next computing revolution. in Proceedings of the 47th design automation conference; 2010.

Zhu L, et al. Synthesis of fluorescent silver nanoclusters: Introducing bottom-up and top-down approaches to nanochemistry in a single laboratory class. Journal of Chemical Education. 2019;97(1):239- 243.

Durán N, Marcato PD. Nanobiotechnology perspectives. Role of nanotechnology in the food industry: a review. International Journal of Food Science & Technology. 2013;48(6):1127-1134.

Yousaf H, et al. Green synthesis of silver nanoparticles and their applications as an alternative antibacterial and antioxidant agents. Materials Science and Engineering: C. 2020;112:110901.

Iravani S, et al., Synthesis of silver nanoparticles: chemical, physical and biological methods. Research in pharmaceutical sciences. 2014;9(6): 385.

Raveendran P, Fu J, Wallen SL. Completely “green” synthesis and stabilization of metal nanoparticles. Journal of the American Chemical Society. 2003;125(46):13940-13941.

Choudhary NL, Chishty N. Effect of Habitat Loss and Anthropogenic activities on butterflies survival: A review. International Journal of Entomology; 2020.

Kumar S, Singh B, Bajpai V. Traditional uses, phytochemistry, quality control and biological activities of genus Grewia. Phytomedicine plus. 2022;2(3):100290.

Shukla S, Salve RV. Development of beverages based on temperate fruits. Magnesium. 2023;4(5.1).

Swain S, et al. Recent progression on phytochemistry and pharmacological activities of Grewia asiatica L.(Tiliaceae) and traditional uses. South African Journal of Botany. 2023;155:274-287.

Thilak B, Riyas CT, Swapna TS. Identification of bioactive compounds from the ethnomedicinal plant Senna alata (L.) Roxb.(fabaceae) through in vitro and molecular docking analysis against?-glucosidase enzyme: a diabetic drug target. Plant Science Today. 2023;10(3):235-249.

Aziz A, Memon Z, Bhutto A. Efficient photocatalytic degradation of industrial wastewater dye by Grewia asiatica mediated zinc oxide nanoparticles. Optik. 2023;272:170352.

El-Saadony MT, et al., Biosynthesis, optimization and characterization of silver nanoparticles using a soil isolate of Bacillus pseudomycoides MT32 and their antifungal activity against some pathogenic fungi. Adv. Anim. Vet. Sci. 2019;7(4):238-249.

Al-Ghamdi AY. Antimicrobial and catalytic activities of green synthesized silver nanoparticles using bay laurel (Laurus nobilis) leaves extract. Journal of Biomaterials and Nanobiotechnology. 2019;10(1):26-39.

Singh A, et al. Synthesis and characterization of nanoparticles used in consumer products. Toxicology of nanoparticles: Insights from Drosophila. 2020:1-27.

Zhou H, McClements DJ. Recent advances in the gastrointestinal fate of organic and inorganic nanoparticles in foods. Nanomaterials. 2022;12(7):1099.

Sathi Khatun M0 Fabrication of cellulose capped MnxOy-Ag nanocomposite and investigation of its oxidative and antibacterial efficacy in water environment; 2021.

Kavitha A, et al. Synthesis and enhanced antibacterial using plant extracts with silver nanoparticles: therapeutic application. Inorganic Chemistry Communications. 2021;134:109045.

Saratale GD, et al. Anti-diabetic potential of silver nanoparticles synthesized with Argyreia nervosa leaf extract high synergistic antibacterial activity with standard antibiotics against foodborne bacteria. Journal of Cluster Science. 2017;28:1709-1727.

Habib A, et al. Experimental and theoretical study of catalytic dye degradation and bactericidal potential of multiple phase Bi and MoS 2 doped SnO 2 quantum dots. RSC advances. 2023;13(16):10861-10872.

Parvekar P, et al. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus. Biomaterial investigations in dentistry. 2020;7(1):105-109.

Lalitha A, Subbaiya R, Ponmurugan P. Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property. Int J Curr Microbiol App Sci. 2013;2(6):228-235.

Li X, et al. Recent progress in determination of ochratoxin a in foods by chromatographic and mass spectrometry methods. Critical reviews in food science and nutrition. 2022;62(20):5444-5461.

Vilchis-Nestor AR, et al. Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. Materials letters. 2008;62(17-18):3103-3105.

Iravani S, Varma RS. Green synthesis, biomedical and biotechnological applications of carbon and graphene quantum dots. A review. Environmental chemistry letters. 2020;18:703-727.

Ali K, et al., Microwave accelerated green synthesis of stable silver nanoparticles with Eucalyptus globulus leaf extract and their antibacterial and antibiofilm activity on clinical isolates. PloS one. 2015;10(7): e0131178.

Chaudhary J, et al. Green route synthesis of metallic nanoparticles using various herbal extracts: A review. Biocatalysis and Agricultural Biotechnology. 2023:102692.

Liaqat N, et al. Green synthesized silver nanoparticles: Optimization, characterization, antimicrobial activity, and cytotoxicity study by hemolysis assay. Frontiers in Chemistry. 2022;10:952006.

Das CA, et al. Antibacterial activity of silver nanoparticles (biosynthesis): A short review on recent advances. Biocatalysis and Agricultural Biotechnology. 2020;27:101593.

Rather MA, et al. Ecofriendly phytofabrication of silver nanoparticles using aqueous extract of Cuphea carthagenensis and their antioxidant potential and antibacterial activity against clinically important human pathogens. Chemosphere. 2022;300: 134497.