Asian Journal of Biochemistry, Genetics and Molecular Biology

This study evaluated the effect of ethanol extract of Persea americana pulp on caffeine induced testicular damage on male wistar rats. Fifty-four (54) mature male rats were used, they were divided into six (6) groups of nine (9) rats. Group 1 was not induced with caffeine, Group two(2) to group six(6) were induced with 200mg/kg caffeine for two weeks. Three rats were sacrificed from each group after two weeks of induction. Semen and  testes collected for total sperm count, sperm motility, sperm morphology, sperm vitality and histopathology. The rats were treated as follows for another four weeks, Group 1: no induction, no treatment. Group 2: induced withcaffeine without treatment. Group 3; 100mg/kg extract. Group 4:300mg/kg extract. Group 5:500mg/kg extract, Group 6:0.107mg/kg proviron. Three rats from each group were sacrificed two weeks after treatment and four weeks after treatment. semen and testes were collected for sperm parameters and histopathology respectively. Different doses of Persea americanapulp was able to improve the sperm quality in rats induced with caffeine in a dose and time dependent manner. Histopathological examination of the testes treated with extract showed recovery from damages caused by caffeine in dose dependent manner. Ethanol extract of Persea americanapulphas a dose ameliorating effect on caffeine induced testicular damage on male rats.