Effects of Aqueous Colocasia esculenta Extracts on Selected Biochemical Parameters in Phenyl Hydrazine Induced Male Anemic Albino Rats
Olaitan Nugboton Samuel *
Cell and Tissue Culture Research Laboratory (Drug Discovery Unit), Department of Biochemistry, Lagos State University, Ojo, P.M.B. 0001, Lagos, Nigeria.
Adekunle Abiodun Ayoade
Cell and Tissue Culture Research Laboratory (Drug Discovery Unit), Department of Biochemistry, Lagos State University, Ojo, P.M.B. 0001, Lagos, Nigeria.
Seyi Adebayo David
Cell and Tissue Culture Research Laboratory (Drug Discovery Unit), Department of Biochemistry, Lagos State University, Ojo, P.M.B. 0001, Lagos, Nigeria.
Adedayo Emmanuel Ogunware
Cell and Tissue Culture Research Laboratory (Drug Discovery Unit), Department of Biochemistry, Lagos State University, Ojo, P.M.B. 0001, Lagos, Nigeria.
Ifeoluwa Israel Odekunle
Cell and Tissue Culture Research Laboratory (Drug Discovery Unit), Department of Biochemistry, Lagos State University, Ojo, P.M.B. 0001, Lagos, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: In this study, the effect of Colocasia esculenta a hematinic plant on biochemical parameters levels (Direct, Total and unconjugated bilirubin, creatinine, total protein, serum albumin and urea) was assessed to determine if the plant extract can reverse the abnormality in the values of these parameter.
Methodology: The experimental animals were divided into four groups as follows; group 1(non-anemic control), group 2 (anemic untreated), group 3 (anemic treated with low dose of plant extract 100 mg/ml), group 4 (anemic treated with high dose of plant extract 500 mg/ml). Anemia was induced in group 1, group 2 and group 3 with 60 mg/kg of phenyl hydrazine for 2 days. After induction of anemia group 2 and group 3 was treated with 100 mg/kg and 500 mg/kg of plant extract for 7 days. After 7-day blood sample was collected through heart puncture and centrifuged for serum. Then, Bilirubin, creatinine, total protein, serum albumin and urea test were carried out.
Results: The anemic untreated group had the highest bilirubin, creatinine and urea value of 1.3 mg/dl, 3.97 mg/dl and 71.78 g/l respectively compared to the non-anemic control (bilirubin-0.4 mg/dl, creatinine-3.13 mg/dl and urea 60.35 mg/dl), anemic treated with low dose (bilirubin-0.37 mg/dl,creatinine-1.40 mg/dl and urea-41.82), and anemic treated with high dose (bilirubin-0.25 mg/dl, creatinine-0.86 mg/dl, and urea-48.66 mg/dl) with significant increase in phenyl hydrazine value at p<0.05 .The anemic nontreated group experienced a reduced value of total protein and albumin of 49.78 g/l and 24.46 g/l respectively compared to the non-anemic control (total protein-66.2 g/l and albumin-37.67 g/l) ,anemic treated with low dose (total protein-67.5 g/l and albumin-19.2 g/l), and anemic treated with high dose (total protein-21.9 g/l and albumin-81.6 g/l).
Conclusion: The obtained results from this study revealed the anti-anemia potentials of aqueous extracts of Colocasia esculenta.
Keywords: Colocasia esculenta, phenyl hydrazine, anemia, bilirubin, creatinine, urea, total protein serum albumin.