Tissue Specific Genomic and Proteomic Profiling in Albino Wistar Rat
Raja Saha
Biodiversity and Environmental Studies Research Centre, Midnapore City College, affiliated to Vidyasagar University, Paschim, Medinipur, West Bengal - 721129, India.
Tuhin Khaddar
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Sanchari Pal
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Arpita Singha
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Anirban Chakraborty
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Riya Samai
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Ipsita Koley
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Santashree Maity
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Tiyasa Roy
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Rajashree Panda
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Rimpi Samanta
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Arijit Chakraborty
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Mitusi Koley
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Debojyoti Pradhan
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Mousumi Shit
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Santipriya Maity
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Sunanda Shit
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Taslima Nasrin
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Daliya Panja
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Priti Roy
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
Sangita Maiti Dutta
*
Department of Biological Sciences, Midnapore City College, Paschim Medinipur, West Bengal - 721129, India.
*Author to whom correspondence should be addressed.
Abstract
Animal tissues from Albino Wistar rats provide a valuable model to compare uniform genomic DNA with tissue-specific protein expression, enabling baseline molecular profiling across organs. This study aimed to isolate high-quality genomic DNA and characterize tissue-specific protein expression profiles from liver, kidney, lung, and brain tissues of albino Wistar rat (Rattus norvegicus). Genomic DNA was extracted using the phenol-chloroform method and evaluated by spectrophotometric analysis and agarose gel electrophoresis. All samples exhibited high purity (A260/A280: 1.80-1.97) and intact high-molecular-weight DNA bands with minimal degradation. Total protein was quantified using the Lowry method, demonstrating tissue-dependent variation, with the highest concentration observed in liver (93 µg/mL), followed by kidney (86 µg/mL), lung (78.6 µg/mL), and brain (76.5 µg/mL). SDS-PAGE analysis revealed distinct and complex banding patterns across tissues, reflecting differential protein expression profiles. Despite uniform genomic integrity across tissues, proteomic heterogeneity was evident, consistent with organ-specific functional specialization. This study provides baseline comparative molecular profiling across rat tissue and establishes reliable protocols for simultaneous genomic and proteomic analysis and provides baseline molecular data for future biomedical and toxicological investigations.
Keywords: Rattus norvegicus, genomic DNA isolation, protein estimation, SDS-PAGE, electrophoresis