Genetic Kinship of Nilem Fish Strains (Osteochillus hasselti)
Yuli Andriani *
Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung – Sumedang Km 21 Jatinangor, Sumedang, Jawa Barat, Indonesia.
Ibnu Dwi Buwono
Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung – Sumedang Km 21 Jatinangor, Sumedang, Jawa Barat, Indonesia.
Ujang Subhan
Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung – Sumedang Km 21 Jatinangor, Sumedang, Jawa Barat, Indonesia.
Arini Mandhasia
Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung – Sumedang Km 21 Jatinangor, Sumedang, Jawa Barat, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
The research aims to analyze the kinship of nilem fish strains using RAPD (Random Amplified Polymorphic DNA). This research used exploratory method with descriptive analysis. The primers used in the RAPD method are OPA-02 (5'-TGCCGAGCTG-3 '), OPA-03 (5'-AGTCAGCCAC-'3), OPA-05 (5'-AGGGGTCTTG-3') and OPA-11 (5) 5'- CAATCGCCGT-3 '). The primary optimization results showed that OPA-02 and OPA-11 were the primary primers for detecting polymorphic and monomorphic fragments of the nilem fish genome. Phenograms from the RAPD method that were processed through the NTSYS-pc program showed that the OPA-02 primer was the best primer for nilem kinship analysis. Relationship between red nilem with green nilem is 92%, red nilem and green nilem with mangot nilem are 72% and the three nilem with beureum panon are 12%.
Keywords: Kinship, RAPD, nilem strain.