Asian Journal of Biochemistry, Genetics and Molecular Biology https://journalajbgmb.com/index.php/AJBGMB <p style="text-align: justify;"><strong>Asian Journal of Biochemistry, Genetics&nbsp;and&nbsp;Molecular Biology (ISSN: 2582-3698)</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/AJBGMB/general-guideline-for-authors">Click here for Types of paper</a>). The area of interest of AJBGMB includes but not restricted to all aspects of&nbsp; Biochemistry,&nbsp;Genetics and Molecular Biology. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN peer-reviewed, open access INTERNATIONAL journal.</p> <p>&nbsp;</p> Asian Journal of Biochemistry, Genetics and Molecular Biology en-US Asian Journal of Biochemistry, Genetics and Molecular Biology 2582-3698 Total Bacteria of Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender https://journalajbgmb.com/index.php/AJBGMB/article/view/30129 <p><strong>Aims:</strong> To determine the effect of the addition of sweet orange essential oil to the extender (tris yolk extender without the addition of antibiotics/antibacterial) to total bacteria Boer Buck frozen semen.</p> <p><strong>Study Design:</strong> Randomized Block Design.</p> <p><strong>Place and Duration of Study:</strong> Sample: Laboratory Reproduction of Loka Penelitian Kambing Potong Sei Putih Indonesia, between January and March 2019.</p> <p><strong>Methodology:</strong> The research procedure starts with the preparation of semen extender, collection of fresh semen, dilution of semen, equilibration, freezing of semen, and thawing. This research obtained conducted using a Randomized Block Design consisting of 5 treatment levels and five replications. Semen storage using 3 Boer buck, which done for three days. As a treatment is the addition of sweet orange essential oil as much as (P0) 0%, (P1) 0,25%, (P2) 0,5%, (P3) 0,75% and (P4) 1% on the tris yolk extender. The observed variables was total bacteria evaluated before freezing and after freezing (Post-Thawing).</p> <p><strong>Results:</strong> The results showed that the addition of sweet orange essential oil had a very significant effect (P &lt;0.01). The results of adding sweet orange essential oil to the extender (Post-Thawing) were 78.6 x 102 CFU (P0), 76 x 102 CFU (P1), 73,2 x 102 CFU (P2), 71 x 102 CFU (P3) and 68.6 x 102 CFU (P4).</p> <p><strong>Conclusion:</strong> The best values the total bacteria 68.6 x 102 CFU (P4). It can conclude that the best result of adding sweet orange essential oil to tris yolk extender is (P4) 1%.</p> Sukma Aditya Sitepu Zaituni Udin . Jaswandi . Hendri ##submission.copyrightStatement## 2020-09-21 2020-09-21 1 5 10.9734/ajbgmb/2020/v5i330129