Asian Journal of Biochemistry, Genetics and Molecular Biology <p style="text-align: justify;"><strong>Asian Journal of Biochemistry, Genetics&nbsp;and&nbsp;Molecular Biology (ISSN: 2582-3698)</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/AJBGMB/general-guideline-for-authors">Click here for Types of paper</a>). The area of interest of AJBGMB includes but not restricted to all aspects of&nbsp; Biochemistry,&nbsp;Genetics and Molecular Biology. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN peer-reviewed, open access INTERNATIONAL journal.</p> <p>&nbsp;</p> en-US (Asian Journal of Biochemistry, Genetics and Molecular Biolog) (Asian Journal of Biochemistry, Genetics and Molecular Biolog) Sat, 05 Sep 2020 06:36:42 +0000 OJS 60 Chemical Analysis of (Lagenaria siceraria) Calabash Seeds Oil <p>Characterization of fats and oils is important in assessing their potential economic uses. This study was carried out to determine and compare the physicochemical characteristics of a commercial oil and solvent extracted oil from the kernels of calabash seeds (<em>Lagenaria siceraria</em><em>)</em>. The range of physicochemical properties are: Solvent extraction recorded a higher yield of (42.3±0.66%) acid value (9.4±1.8 - 8.2±0.5 mgKOH/g of oil), iodine value (4.56±2.6 - 4.46±0.07 mg/100g), saponification value (162.7±4.58 - 159.42±3.68 mgKOH/g), peroxide value (6.0±1.63 - 5.3±2.4 mEq/kg), free fatty acid (10.3±1.76 - 9.89±1.8 % as oleic acid). The low iodine value indicates a high degree of saturation of the fat as well as its non-drying nature.</p> M. Hashimu, J. Ibrahim, S. Y. Lema, J. Suleiman ##submission.copyrightStatement## Fri, 11 Sep 2020 00:00:00 +0000 Determination of LD50, Fecundity and Locomotor Effects of Methanol Root Extract of Ximenia americana Linn, in Drosophila melanogaster <p><strong>Aim:</strong> The study aimed at assessing the LD<sub>50</sub>, fecundity and locomotor effects of <em>Ximenia americana</em> L in <em>Drosophila melanogaster.</em></p> <p><strong>Study Design: </strong>The study was an experimental design.</p> <p><strong>Place and Duration of Study: </strong>The study was done in the drosophila laboratory, Africa Centre of Excellence in Phytomedicine Research and Development (ACEPRD), University of Jos, Nigeria between November 2019 and Match, 2020.</p> <p><strong>Methods: </strong>The experimental animals (1-3 days old) of both sexes were exposed to different concentrations (1 mg 10 mg 50 mg, 100 mg, 200 mg, 250 mg 300 mg, 350 mg, 400 mg, 450 mg) of the plant extract per oral for seven days to determine the lethal dose, LD<sub>50</sub>. Thereafter, five days treatment was done using 50 mg, 100 mg 200 mg and 300 mg concentrations of the extract to assay for fecundity and locomotor effect in the fruit fly.</p> <p><strong>Results:</strong> The LD<sub>50 </sub>of the methanol extract of <em>Ximenia americana </em>in <em>D. melanogaster</em> was found to be 327.7 mg, this showed that the plant extract is relatively safe. Also, the result showed that both fecundity and locomotor behaviour of the treated and untreated flies was not significantly (<em>P</em> &gt; 0.05) different. Thus, the extract at the used concentrations does not affects significantly both the reproductive capacity and the motor functions in the fruit fly.</p> <p><strong>Conclusion:</strong> All the tested concentrations used in this research are relatively safe (because of high LD<sub>50</sub> 327.7 mg) in the fruit fly and slightly increase the emergence of new fly with no noticeable negative effect in locomotor activity.</p> Great David Bagu, Simeon Omale, Walter Mdekera Iorjiim, Mary O. Uguru, Steven Samuel Gyang ##submission.copyrightStatement## Sat, 05 Sep 2020 00:00:00 +0000 Invitro Antimalarial and Antibacterial Activities of Methanol Stem Bark Extract of Frankincense Tree (Boswellia dalzielii) and Leaves Extract of Kenaf (Hibiscus cannabinus) <p><strong>Aim:</strong> This research investigated the anti-malarial and antibacterial activities of stem bark methanol extract of Frankincense tree and Kenaf leaves extract on <em>Plasmodium falciparum</em> parasite and against five clinically significant bacteria.</p> <p><strong>Study Design: </strong>Laboratory-experimental design was used for this study.</p> <p><strong>Place and Duration of Study:</strong> This study was carried out between September 2019 and November</p> <p>2019 at Biochemistry and Microbiology laboratories, Sokoto State University, Sokoto, Nigeria.</p> <p><strong>Methodology:</strong> The <em>in vitro</em> antimalarial activity test was conducted by determining the parasitemia for each sample concentration by manual counting on thin Giemsa smears after a 24-hour incubation with the extracts in order to determine the IC<sub>50</sub> values. The antibacterial study was done using a modified agar well diffusion technique.</p> <p><strong>Results: </strong>The testing revealed that methanol stem bark extract of Frankincense tree and Kenaf leaves exhibited good and potent (very good) antimalarial activities against <em>P. falciparum</em> respectively with IC<sub>50</sub> values of 1.25 μg/mL and 0.16 μg/mL respectively. They also show good inhibitory activities against <em>E.coli</em> but slight inhibitory activities against the other pathogens tested.</p> <p><strong>Conclusion:</strong> The current study indicates that extracts of these plants exhibit anti-malarial and antibacterial activities and may serve as useful sources of drugs for treatment of malaria caused by <em>P. falciparum parasite</em> as well as bacterial infections caused by the tested bacteria.</p> S. Salihu, C. A. Otitolaiye, M. U. Hizbullah ##submission.copyrightStatement## Wed, 09 Sep 2020 00:00:00 +0000 Evaluation of Influence of Archachatina marginata Mucin on the Body Weight and Some Gastric Secretions of Wistar Rats Induced with Gastric Ulcer <p>The aim of this study was to evaluate the influence of<em> A. marginata</em> mucin on the gastric secretions and body weight of wistar rats induced with gastric ulcer. Thirty (30) adult male wistar rats were divided into six groups of five rats each. Group I (normal control) was administered with 2ml/kg distilled water, while Groups II III, IV and V were induced with ulceration via oral administration of 120mg/kg indomethacin. After ulceration had been confirmed successfully induced, while Group II was left untreated with mucin, III, IV and V were treated with 200mg/kg, 400mg/kg and 800mgl/kg mucin respectively. Meanwhile, Group VI was administered with 20mg/kg omeprazole (standard drug). Body weight of rats was determined after induction (initial) and after treatment (final).&nbsp; The values recorded for free acidity (FA), total acidity (TA), pepsin activity (PA) and acid output (AO) for the untreated ulcer rats (Group II) were significantly high (63.20±7.66mEq/L), (91.40±6.34mEq/L), (1.63±0.09μEq/L/4hr) and (0.43±0.04μg/ml) respectively. However, significant reduction in the aforementioned parameters was established with mucin treatment in a dose dependent manner, such that Group V administered with 800mg/kg manifested the lowest level of FA (47.40±7.19 mEq/L), TA (72.00±6.36), PA (1.39±0.07 μg/ml) and AO (0.16±0.05 μg/ml) which was not significantly different from the values recorded for Group VI treated with the standard drug (omeprazole).The values recorded on the body weight of Group II reduced with the induction of gastric ulcer. However, a dose dependent increase was observed in all treated groups. In conclusion, through this study, it has been established that <em>A. marginata</em> mucin could be explored for the development of anti-ulcer therapy.</p> Rizwan, A. Ansari, Karimah, Mohammad Rabiu, Oludunni Otanwa Omolabake, Elendu Melford Uche ##submission.copyrightStatement## Sat, 12 Sep 2020 00:00:00 +0000 Intracellular Protein Biosynthesis: A Review <p>Proteins are macromolecules made up of many amino acids that linked together by peptide bond to make a protein molecule. The sequence and the number of amino acids determines each protein unique structure and specific function. Proteins play a vital role in living systems and play important biological functions. Biosynthesis of protein occur in our body cells in order to support the biological function in our body. Intracellular protein synthesis is a complex process that involve the transformation of information and instructions from a genetic material DNA inside the nucleus to form mRNA molecules that transferred to the cytoplasm and liked to the cytoplasmic ribosome. Subsequently, the m RNA and further encode a sequence of amino acid in a specific order and number to form a polypeptide chains that finally undergoes conformational changes and folding to form a particular structure protein. This review will focus on the tow consecutive stages of protein biosynthesis; transcription and translation, and their substage processes; initiation, elongation, and termination. Briefly, overview the role of protein in the biological function and the different types of protein structure.</p> Abdulsalam Alhalmi, Nafaa Alzobaidi, Amer Abdulrahman ##submission.copyrightStatement## Tue, 08 Sep 2020 00:00:00 +0000