Open Access Original Research Article

Identifying Genetic Diversity of Cotton Leaf Curl Virus in Commercial Cotton (Gossypium hirsutum L.)

Syed Bilal Hussain, Maria Rizwi, Tahir Naqqash, Muhammad Zubair, Samreen Sarwar

Asian Journal of Biochemistry, Genetics and Molecular Biology, Page 1-9
DOI: 10.9734/ajbgmb/2021/v9i430223

Plant viruses have become a major issue to the crop production around the world. These viruses have become an unavoidable limiting factor by causing rigorous crop deficit for those major crops which are economically important. Among these viruses, begomoviruses belonging to family Geminiviridae, have brought the major devastation to both monocots and dicots in tropical and sub-tropical regions. These begomoviruses are transmitted through white fly and cause diseases like mosaic, yellow mosaic, yellow leaf curling, and yellow vein etc. The objective of this study is to evaluate the association between the genetic diversity of begomoviruses and phenotypic data to identify the ideal cotton genotype for breeding. In this study, 30 leaf samples with CLCuD symptoms were analyzed using rolling circular amplification and PCR. Obtained results expressed the presence of begomovirus with its associated satellites (i.e. alpha and betasatellites) in six varieties, association of begomovirus and betasatellites in four varieties and combination of begomovirus with alphasatellite in six varieties. Severe disease symptoms were exhibited with these combinations in selected varieties but betasatellite-begomovirus complex demonstrated a deadly impact. Few varieties expressed the resistance against the begomoviruses, so these varieties can further be manipulated for breeding.

Open Access Original Research Article

Study of Genetic Analysis of Physiological Attributes under Normal and Drought Conditions in Gossypium hirsutum L.

Muhammad Akbar, Syed Bilal Hussain, Farzana Ashraf, Muhammad Zubair

Asian Journal of Biochemistry, Genetics and Molecular Biology, Page 10-22
DOI: 10.9734/ajbgmb/2021/v9i430224

The aim of this study was to identify the suprior cotton genotypes with improved physiological characteristics under drought conditions. On the bais of root-shoot charcteristics, five genoypes of  G. hirsutum were identified as drought tolerant and three genotypes screened out as drought susceptible. The field screening experiment were carried out to validate the findigs of root-shoot screening sudy on the basis of physiological as well as agronomical chracteristics. Both drought tolerant and susceptible genotypes were grown in glass house in pots and followed line x tester mating desighn to cross these genotyps. Parents with their fiftenn offsprings were grown in field conditions in very next cotton season for further analysis. Analysis of variance showed the existence of significant variations among the accessions for all the physiological parameters i.e, osmotic potential, relative water content, cell injury, leaf water potential, excised leaf water loss, stomatal conductance, photosynthesis rate and transpiration rate. Further, among parents line, MS-64 and tester, BH-176 showed superior performance under water scarce conditions. Among the crosses COOKER-315 x Cyto-62 and GS-444 x MPS-11 were better perfomed for high yielding parametrs. The results showed that these two combinations might be helpful to develop drought resistant germplasm on large scale.

Open Access Original Research Article

Polymorphic Investigation of ADCY2 and C7 Genes in Local Population of Oral Cancer

Syeda Zahra Abbas, Ayesha Altaf, Syed Aun Muhammad, Tahir Naqqash, Muhammad Zubair, Syed Bilal Hussain, Tahira Qousain Naqvi, Husnain Raza, Saqib Khan

Asian Journal of Biochemistry, Genetics and Molecular Biology, Page 23-33
DOI: 10.9734/ajbgmb/2021/v9i430225

Single nucleotide polymorphisms (SNP) are responsible for genetic mutations. We studied genetic molecular variations and found an association of oral cancer with SNP of Adenylate Cyclase 2 (ADCY2) rs252546 and complement C7 (C7) rs1450656 genes in people of Southern Punjab, Pakistan. The study involves 100 cases of oral cancer and 100 healthy individuals. ADCY2 is found as a membrane-associated enzyme and C7 is involved in innate immunity. The process of genotyping was carried out by Tetra ARMS Primer PCR. The genetic variant of ADCY2 rs252546 has allelic origin G/A and C7 rs1450656 with C/T. The statistical analysis showed that the 51-60 years age group is significantly associated with oral cancer. The allelic frequency of ADCY2 rs252546 and C7 rs1450656 was calculated through Hardy Weinberg equilibrium. The homozygous mutant allele G of ADCY2 was more prevalent in cases and C allelic genotype was equally found in cases and controls. Other demographic and polymorphic studies indicated a significant association of variants of ADCY2 and C7 with oral cancer in the local population of Punjab. Variations in ADCY2 and C7 can be used as potential biomarkers and biological targets for oral cancer management strategies.