Hypoglycemic Potential of Dietary Supplementation of Protein Isolate from Fermented Cucumeropsis manii in Streptozotocin Induced Hyperglycemic in Male Wistar Albino Rats
A. O. Abiola
Department of Biochemistry, Lagos State University, Nigeria.
A. O. Iyoribhe
Department of Biochemistry, Lagos State University, Nigeria.
S. A. Adeniyi
Department of Biochemistry, Lagos State University, Nigeria.
O. B. Adu
Department of Biochemistry, Lagos State University, Nigeria.
A. S. Ogunbowale
Department of Biochemistry, Lagos State University, Nigeria.
P. A. Adedigba
Department of Biochemistry, Lagos State University, Nigeria.
D. B. Awojobi
Department of Biochemistry, Lagos State University, Nigeria.
T. M. Johnson
Department of Biochemistry, Lagos State University, Nigeria.
J. O. Oyedola
Department of Biochemistry, Lagos State University, Nigeria.
T. O. Wahab
Department of Biochemistry, Lagos State University, Nigeria.
S. F. Ajose
Department of Biochemistry, Lagos State University, Nigeria.
B. O. Elemo *
Department of Biochemistry, Lagos State University, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The effect of Protein isolate from fermented melon seeds (Ogiri Protei Isolates; OPI) of Cucumeropsis manii on blood glucose, lipid profile, and antioxidant enzyme activities in streptozotocin (STZ)-induced diabetic rats was investigated. Thirty Male Wistar rats were divided into five equal groups. GThe first control group with no exposure. The second group of rats with Streptozotocin-induced non-treated diabetes. The 3rd and 4th groups of rats with Streptozotocin-induced diabetes supplemented with Ogiri protein isolates (200, 600 mg/kg in diet). And the 5th group of rats with Streptozotocin-induced diabetes administered glibenclamide in a dose 500 ug/kg in diet [17]. The OPI was administered for 6 weeks. The administration of OPI reduced the blood glucose concentration of the STZ-induced diabetic rats. Sera and hepatic superoxide dismutase, activities of the STZ-induced diabetic rats were significantly (P< 0.05) increased in comparison with the diabetic control rats. Lipid peroxidation of the supplemented OPI diabetic rats was significantly (P< 0.05) decreased in comparison with the diabetic control rats as the administration of OPI to the STZ-induced diabetic rats significantly increased the enzymes’ activities. The concentration of low-density lipoproteins in the OPI supplemented rats was significantly elevated. These data demonstrate that OPI supplements might be beneficial for correcting hyperglycemia but the consumption of OPI can modulate some tissue lipids in a direction not beneficial for CVD risk in patients with diabetes.
Keywords: Ogiri, Ogiri protein isolate, streptozotocin, glibenclamide, antidiabetic activity, hypolipidemic activity, antioxidant activity