Genetic Analysis of (Oreochromis niloticus) Nirvana Tilapia Cultivated in Wanayasa and Galunggung, Lumajang, Bali, Minahasa Using Random Amplified Polymorphic DNA Method
Ayi Yustiati *
Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, 45363, Jatinangor, West Java, Indonesia.
Ibnu Bangkit Bioshina Suryadi
Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, 45363, Jatinangor, West Java, Indonesia.
. Rosidah
Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, 45363, Jatinangor, West Java, Indonesia.
Walim Lili
Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, 45363, Jatinangor, West Java, Indonesia.
Dian Yuni Pratiwi
Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, 45363, Jatinangor, West Java, Indonesia.
Anandita Rahmania
Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, 45363, Jatinangor, West Java, Indonesia.
Maryam Nur Latifah
Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, 45363, Jatinangor, West Java, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
This research aims to determine the kinship of nirvana tilapia (Oreochromis niloticus) cultivated in Wanayasa with Galunggung, Lumajang, Bali, and Minahasa by Random Amplified Polymorphic DNA method. All tilapia cultivated in these four places originated from Wanayasa. This research was conducted in the Biotechnology Laboratory, Laboratory of Microbiology and Molecular Biotechnology, FPIK UNPAD. The research method was an explorative experiment with the RAPD-PCR technique using primary OPA-3 and OPA-5. However, the results of DNA amplification with OPA-3 were better than with OPA-5. Therefore, further analysis was carried out using data from OPA-3. Kinship was analyzed based on the similarity index which was calculated by Numerical Taxonomy and Multivariate Analysis System (NTSYS) program. The results showed that the kinship between Nirvana tilapia cultivated in Wanayasa with Galunggung, Lumajang, Bali, and Minahasa, according to the similarity index was high, about 77-85%. The highest similarity index (85%) was obtained between nirvana tilapia cultivated in Wanayasa with Galunggung, and the lowest similarity index (77%) between tilapia cultivated in Bali with Minahasa. Genetic changes that occur because of differences in cultivation environments range from 15-23%.
Keywords: Tilapia, RAPD-PCR, Nirvana, Oreochromis niloticus, Wanayasa, Minahasa, Bali, Lumajang, Galunggung